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首页> 外文会议>Optical biopsy XV: Toward real-time spectroscopic imaging and diagnosis >Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections
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Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections

机译：幼斑马鱼软组织的同步微显微镜成像显示视网膜-顶盖投影

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摘要

Biomedical research and clinical diagnosis would benefit greatly from full volume determinations of anatomical phenotype. Comprehensive tools for morphological phenotyping are central for the emerging field of phenomics, which requires high-throughput, systematic, accurate, and reproducible data collection from organisms affected by genetic, disease, or environmental variables. Theoretically, complete anatomical phenotyping requires the assessment of every cell type in the whole organism, but this ideal is presently untenable due to the lack of an unbiased 3D imaging method that allows histopathological assessment of any cell type despite optical opacity. Histopathology, the current clinical standard for diagnostic phenotyping, involves the microscopic study of tissue sections to assess qualitative aspects of tissue architecture, disease mechanisms, and physiological state. However, quantitative features of tissue architecture such as cellular composition and cell counting in tissue volumes can only be approximated due to characteristics of tissue sectioning, including incomplete sampling and the constraints of 2D imaging of 5 micron thick tissue slabs. We have used a small, vertebrate organism, the zebrafish, to test the potential of microCT for systematic macroscopic and microscopic morphological phenotyping. While cell resolution is routinely achieved using methods such as light sheet fluorescence microscopy and optical tomography, these methods do not provide the pancellular perspective characteristic of histology, and are constrained by the limited penetration of visible light through pigmented and opaque specimens, as characterizes zebrafish juveniles. Here, we provide an example of neuroanatomy that can be studied by microCT of stained soft tissue at 1.43 micron isotropic voxel resolution. We conclude that synchrotron microCT is a form of 3D imaging that may potentially be adopted towards more reproducible, large-scale, morphological phenotyping of optically opaque tissues. Further development of soft tissue microCT, visualization and quantitative tools will enhance its utility.
机译：生物医学研究和临床诊断将从解剖表型的全体积测定中大大受益。形态学表型的综合工具是新兴的表型学领域的核心，它需要从受遗传，疾病或环境变量影响的生物体中进行高通量，系统，准确和可重复的数据收集。从理论上讲，完整的解剖表型需要评估整个生物体中的每种细胞类型，但是由于缺乏无偏的3D成像方法（尽管光学不透明，该方法可以对任何细胞类型进行组织病理学评估），因此目前这一理想难以实现。组织病理学是当前诊断表型的临床标准，涉及组织切片的微观研究，以评估组织结构，疾病机制和生理状态的定性方面。然而，由于组织切片的特征，包括不完整的采样和5微米厚组织板的2D成像限制，组织结构的定量特征（如细胞组成和组织体积中的细胞计数）只能近似估算。我们使用了一种小的脊椎动物生物斑马鱼来测试microCT在系统宏观和微观形态表型分析中的潜力。虽然通常使用光片荧光显微镜和光学断层扫描等方法来实现细胞分辨率，但是这些方法不能提供组织学的全细胞透视特征，并且受斑马鱼幼体的表征，可见光只能通过有色和不透明的标本进行有限的穿透。 。在这里，我们提供了一个神经解剖的例子，可以通过在1.43微米各向同性体素分辨率下对染色的软组织进行microCT研究。我们得出结论，同步加速器microCT是3D成像的一种形式，可能会被采用来实现光学不透明组织的更可再现，大规模，形态学表型化。软组织microCT，可视化和定量工具的进一步发展将增强其实用性。

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  • 会议地点 San Francisco(US)
  • 作者

    Xuying Xin; Darin Clark; Khai Chung Ang; Damian B. van Rossum; Jean Copper; Xianghui Xiao; Patrick J. La Riviere; Keith C. Cheng;

  • 作者单位

    Department of Pathology, Penn State College of Medicine, Hershey, PA 17033, USA,Jake Gittlen Laboratories for Cancer Research, Hershey, PA 17033, USA,Penn State Consortium for Interdisciplinary Image Informatics and Visualization, USA;

    Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC 27708, USA;

    Department of Pathology, Penn State College of Medicine, Hershey, PA 17033, USA,Jake Gittlen Laboratories for Cancer Research, Hershey, PA 17033, USA,Penn State Consortium for Interdisciplinary Image Informatics and Visualization, USA;

    Department of Pathology, Penn State College of Medicine, Hershey, PA 17033, USA,Jake Gittlen Laboratories for Cancer Research, Hershey, PA 17033, USA,Penn State Consortium for Interdisciplinary Image Informatics and Visualization, USA;

    Department of Pathology, Penn State College of Medicine, Hershey, PA 17033, USA,Jake Gittlen Laboratories for Cancer Research, Hershey, PA 17033, USA,Penn State Consortium for Interdisciplinary Image Informatics and Visualization, USA;

    Advanced Photon Source, Argonne National Laboratory Argonne, IL 60439, USA;

    Department of Radiology, University of Chicago, Chicago, IL 60637, USA;

    Department of Pathology, Penn State College of Medicine, Hershey, PA 17033, USA,Jake Gittlen Laboratories for Cancer Research, Hershey, PA 17033, USA,Penn State Consortium for Interdisciplinary Image Informatics and Visualization, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    zebrafish; phenomics; 3D imaging; image visualization; soft tissue imaging; optic nerves; retinotectal projections; synchrotron microCT;

    机译：斑马鱼词组学3D成像；图像可视化；软组织成像；视神经视网膜直肠投射；同步加速微CT;

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