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Noncontact phase-sensitive dynamic optical coherence elastography at megahertz rate

机译:兆赫兹速率的非接触相敏动态光学相干弹性成像

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Dynamic optical coherence elastography (OCE) techniques have shown great promise at quantitatively obtaining the biomechanical properties of tissue. However, the majority of these techniques have required multiple temporal OCT acquisitions (M-B mode) and corresponding excitations, which lead to clinically unfeasible acquisition times and potential tissue damage. Furthermore, the large data sets and extended laser exposures hinder their translation to the clinic, where patient discomfort and safety are critical criteria. In this work we demonstrate noncontact true kilohertz frame-rate dynamic optical coherence elastography by directly imaging a focused air-pulse induced elastic wave with a home-built phase-sensitive OCE system based on a 4X buffered Fourier Domain Mode Locked swept source laser with an A-scan rate of~1.5 MHz. The elastic wave was imaged at a frame rate of ~7.3 kHz using only a single excitation. In contrast to previous techniques, successive B-scans were acquired over the measurement region (B-M mode) in this work. The feasibility of this method was validated by quantifying the elasticity of tissue-mimicking agar phantoms as well as porcine corneas ex vivo at different intraocular pressures. The results demonstrate that this method can acquire a depth-resolved elastogram in milliseconds. The reduced data set enabled a rapid elasticity assessment, and the ultra-fast acquisition speed allowed for a clinically safe laser exposure to the cornea.
机译:动态光学相干弹性成像(OCE)技术在定量获得组织的生物力学特性方面显示出巨大的希望。但是,这些技术中的大多数都需要多个时间OCT采集(M-B模式)和相应的激发,这导致临床上不可行的采集时间和潜在的组织损伤。此外,庞大的数据集和长时间的激光照射阻碍了它们向诊所的翻译,在这里,患者的不适和安全性是关键标准。在这项工作中,我们通过使用基于4X缓冲傅里叶域锁模扫频源激光器的家用相敏OCE系统直接成像聚焦的空气脉冲感应的弹性波,展示了非接触式真正的千赫兹帧频动态光学相干弹性成像。 A扫描速率约为1.5 MHz。仅使用一次激励就以约7.3 kHz的帧频对弹性波成像。与以前的技术相比,这项工作在测量区域(B-M模式)上进行了连续的B扫描。通过定量模拟在不同眼压下离体的组织琼脂模体以及猪角膜的弹性,验证了该方法的可行性。结果表明,该方法可以在几毫秒内获得深度解析的弹性图。减少的数据集可以进行快速的弹性评估,并且超快的采集速度允许临床安全地将激光暴露于角膜。

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