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首页> 外文会议>Optical interactions with tissue and cells XXVI >Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte.
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Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyte.

机译：他拉泊芬钠对心肌细胞的细胞外光敏反应中白蛋白结合的细胞毒性变化。

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摘要

We studied the myocardial cell necrosis measuring cell lethality with various albumin concentrations against oxidative stress by extracellular photosensitization reaction using talaporfin sodium. We have proposed to induce the photosensitization reaction in interstitial space of myocardium with short drug-light interval to realize the immediate cell damage by myocardial cell membrane for tachyarrhythmia treatment. To understand the effect of the particular extracellular photosensitization reaction in interstitial space, we measured the myocardial cell lethality 2 hours after the photosensitization reaction by WST assay varying the talaporfin sodium concentration, radiant exposure, and wide albumin concentration of 0-15 mg/ml. The cell lethality was decreased with albumin concentration increasing over 85 % in binding ratio between talaporfin sodium and albumin. The cell lethality didn't change between 0-85% in binding ratio between talaporfin sodium and albumin. The calculated deposited energy to the talaporfin sodium solution was nearly constant of 8.4±1.6 J/well in average varying albumin concentration. We think the cell killing effect by the extracellular photosensitization reaction has a threshold between 85-100% in the binding ratio between talaporfin sodium and albumin in the case of the talaporfin sodium concentration of 40 μg/ml and radiant exposure of 0-40 J/cm~2.
机译：我们研究了使用塔拉泊芬钠通过细胞外光敏反应测量不同白蛋白浓度对氧化应激的细胞致死率的心肌细胞坏死。我们提出了在药物间隔短的情况下在心肌间隙中诱导光敏反应，以实现心肌细胞膜对快速性心律失常治疗的直接细胞损伤。为了了解特定的细胞外光敏反应在组织间隙中的作用，我们通过WST分析测定了光敏反应2小时后心肌细胞的致死率，改变了他拉泊芬钠浓度，辐射暴露和0-15 mg / ml的宽白蛋白浓度。塔拉泊芬钠与白蛋白之间的结合率随着白蛋白浓度的增加超过85％而降低了细胞的致死性。他拉泊芬钠与白蛋白的结合率在0-85％之间没有变化。计算出的他拉泊芬钠溶液的沉积能量在平均变化的白蛋白浓度下几乎恒定为8.4±1.6 J /孔。我们认为在塔拉泊芬钠浓度为40μg/ ml且辐射暴露为0-40 J /的情况下，细胞外光敏反应对细胞的杀伤作用在他拉泊芬钠和白蛋白之间的结合率上具有85-100％的阈值厘米〜2。

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  • 来源
    《Optical interactions with tissue and cells XXVI》|2015年|93210I.1-93210I.4|共4页
  • 会议地点 San Francisco CA(US)
  • 作者

    Emiyu Ogawa; Sayaka Motohashi; Tsunenori Arai;

  • 作者单位

    School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama City, Kanagawa, 223-8522, Japan;

    School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama City, Kanagawa, 223-8522, Japan;

    School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama City, Kanagawa, 223-8522, Japan;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    NPe6; Laserphyrin; myocardial cell;

    机译：NPe6;激光卟啉;心肌细胞;

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