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Near-field scanning optical microscope with an optically tra

机译：具有光学镜架的近场扫描光学显微镜

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Abstract: We developed a near-field scanning optical microscope (NSOM) which uses an optically trapped metallic Rayleigh particle as a near-field probe. This microscope is suitable for observations of biological specimen under aqueous condition because the probe particle is held by non-contact force from radiation pressure. The spring constant of trapping force is so weak (several mN/m order typically) that induced damage on a sample rarely occurs by this force. We used a gold colloidal particle which had a diameter of 40 nm as a probe. The scattering efficiency of the particle (at 488-nm wavelength, n$-Au$/ equals 0.916, K$-Au$/ equals 1.840) is 348 times as great as that of a glass particle (n$-glass$/ equals 1.5) in the same size under water. We investigated a surface of a cover glass with the gold colloidal particle and confirmed the ability to observe small irregularities of the surface in around 10 nm. We also observed aggregated gold colloidal particles (d equals 40 nm) on a cover glass and fluorescent beads on glass. The investigation of DNA stained with YOYO-1 iodide on glass under water conditions was also performed. !33
机译：摘要：我们开发了一种近场扫描光学显微镜（NSOM），该显微镜使用光学捕获的金属瑞利粒子作为近场探针。这种显微镜适用于在水性条件下观察生物样本，因为探针粒子是由辐射压力的非接触力保持的。捕获力的弹簧常数太弱（通常为几个mN / m量级），以致这种力很少会引起样品损坏。我们使用直径为40 nm的金胶体颗粒作为探针。粒子的散射效率（在488 nm波长下，n $ -Au $ /等于0.916，K $ -Au $ /等于1.840）是玻璃粒子（n $ -glass $ /等于）的348倍1.5）在相同大小的水下。我们用金胶体颗粒研究了盖玻片的表面，并确认了在10 nm左右观察到表面不规则现象的能力。我们还观察到在盖玻片上聚集了金胶体颗粒（d等于40 nm），在玻璃上观察到了荧光珠。还进行了在水条件下在玻璃上用YOYO-1碘化物染色的DNA的研究。 ！33

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