Focused ion beams and life science applications: cell tomography and biomachining at ultrahigh resolution

机译：聚焦离子束和生命科学应用：超高分辨率的细胞层析成像和生物加工

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Abstract: A new technique of Focused Ion Beam (FIB) microscopy-nanomachining is proposed for life sciences. Itsperformances are compared with those of currentlyavailable ultramicroscopy apparatuses. Ultra-highresolution tridimensional tomography can be performedon whole cells without preparation. This can beachieved by sequentially etching layers of material andsubsequently viewing the result of the operation undera different perspective. Very fast imaging times(minutes) allow quasi real time microscopy. Thecomplementary technique of nano-biology can beperformed on the same apparatus. The use of the ionbeam allows to imaging both the surface and the innerpart of the sample along any desired plane that can bechosen while the observation is on. !8

机译：摘要：提出了一种用于生命科学的聚焦离子束（FIB）显微镜纳米加工新技术。将其性能与当前可用的超显微仪器进行比较。无需准备就可以对整个细胞进行超高分辨率三维断层扫描。通过依次蚀刻材料层并随后以不同的视角查看操作结果，可以解决这一问题。非常快的成像时间（分钟）允许准实时显微镜检查。纳米生物学的补充技术可以在同一仪器上进行。离子束的使用允许沿观察时可以选择的任何所需平面成像样品的表面和内部。！8

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来源
《Optical Investigations of Cells In Vitro and In Vivo》|1998年|p.221-230|共10页
会议地点 San Jose CA(US)
作者
Author(s): Monica Ballerini Univ. of Milano Milano Italy; Marziale Milani Univ. of Milano (Italy) and INFM Milano Italy; Michele Costato Univ. of Modena Modena MO Italy; I. C. Edmund Turcu Rutherford Appleton Lab. Chilton Didcot Oxon United Ki;
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中图分类细胞生物学;
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机译：将X射线纳米断层扫描与聚焦离子束串行截面成像组合：相关断层扫描在集成电路中的应用
4. Focused ion beams and life science applications: cell tomography andbiomachining at ultrahigh resolution, [C] . Monica Ballerini, Univ. of Milano, Milano, Optical Investigations of Cells In Vitro and In Vivo . 1998

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7. Three-Dimensional Microstructural Visualization of Mitosis using Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) and 3Mv Ultra-High Voltage Electron Microscope (UHVEM) Tomography with Nanoscale Resolution at Whole Cell Level [O] . Iwane Atsuko H., Ohta Keisuke 2015

机译：使用聚焦离子束扫描电子显微镜（FIB-SEM）和3Mv超高压电子显微镜（UHVEM）层析成像技术在全细胞水平上对纳米粒子的有丝分裂进行三维微观结构可视化
8. New High Resolution Scanning Ion Microprobe and Focused Ion Beam Applications [R] . Levi-Setti, R. 1984

机译：新型高分辨率扫描离子微探针和聚焦离子束应用

Focused ion beams and life science applications: cell tomography and biomachining at ultrahigh resolution

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