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首页> 外文会议>Optical Investigations of Cells In Vitro and In Vivo >Imaging of rotational Brownian motion of biomolecules in living cells using fluorescence depolarization microscopy
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Imaging of rotational Brownian motion of biomolecules in living cells using fluorescence depolarization microscopy

机译：使用荧光去极化显微镜成像活细胞中生物分子的旋转布朗运动

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Abstract: Two-dimensional fluorescence depolarization measurement was achieved by a fluorescence microscope equipped with polarizing devices and an image intensified CCD camera. Anisotropy images were acquired using living cells stained with a membrane specific binding dye. It was found that the bound dye is spatially distinguishable from the free dye which does not bind to the membrane of a cell, due to the difference in rotational Brownian motion. In addition, we succeeded in obtaining fluorescence depolarization images by means of time- resolved measurement and two-photon excitation measurement. Two-photon excitation images showed a superior signal-to-noise ratio compared to one-photon excitation images. Fluorescence depolarization imaging will therefore prove a powerful tool for studying molecular functions in cells. !25
机译：摘要：配备偏振镜和图像增强CCD相机的荧光显微镜可实现二维荧光去偏振测量。使用膜特异性结合染料染色的活细胞获取各向异性图像。发现由于旋转布朗运动的差异，结合的染料在空间上可与不结合至细胞膜的游离染料区分开。此外，我们通过时间分辨测量和双光子激发测量成功获得了荧光去极化图像。与单光子激发图像相比，双光子激发图像显示出更高的信噪比。因此，荧光去极化成像将成为研究细胞分子功能的有力工具。 ！25

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