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首页> 外文会议>Optical Investigations of Cells In Vitro and In Vivo >Development of visible diode-laser/fiber-optic colorimetric system for the simple and inexpensive quantification of a protein
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Development of visible diode-laser/fiber-optic colorimetric system for the simple and inexpensive quantification of a protein

机译：可见二极管激光/光纤比色系统的开发，用于简单，廉价地定量蛋白质

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Abstract: A simple and inexpensive quantitative detection of protein in one droplet solution was achieved by a portable diode-laser- based-optical-fiber absorption spectrometry. A system consisted of a visible diode laser, a photodiode and a pair of optical fibers. The conventional Lowry method was used to measure the blue-colored protein solutions. In this study, one droplet of sample, which filled up the void between the end surfaces of two optical fibers, served as a sample cell. The volume of a droplet was 50 $mu@L, which is 1/100 of the cuvette of UV/VIS spectrometer cell used in Lowry method. Although the required sample volume decreased up to 100 times, the detection range was comparable to that of conventional Lowry method in the range of g/L - mg/L. !14
机译：摘要：通过便携式的基于二极管激光的光纤吸收光谱法实现了一种简单且廉价的定量检测液滴溶液中蛋白质的方法。一个系统由可见二极管激光器，光电二极管和一对光纤组成。常规的Lowry方法用于测量蓝色蛋白质溶液。在这项研究中，一滴样品填充了两个光纤端面之间的空隙，作为样品池。液滴的体积为50μL/ L，是Lowry法中使用的UV / VIS光谱仪池的比色杯的1/100。尽管所需的样品量减少了100倍，但在g / L-mg / L的范围内，检测范围与常规Lowry方法相当。 ！14

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