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首页> 外文会议>Optical Investigations of Cells In Vitro and In Vivo >Selective accumulation of meso-tetra(hydroxyphenyl)chlorin in steroid-synthesizing cells of the rat adrenal gland
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Selective accumulation of meso-tetra(hydroxyphenyl)chlorin in steroid-synthesizing cells of the rat adrenal gland

机译：大鼠肾上腺类固醇合成细胞中中四（羟苯基）氯的选择性积累

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Abstract: Rat adrenal glands fluoresce intensely after systemicapplication of meso-tetra(hydroxyphenyl)chlorin(mTHPC). We investigated which parts of the adrenalgland accumulate mTHPC. Furthermore we examined thetime course of adrenal mTHPC-accumulation. Ten maleWistar rats each were given 0.5 or 0.7 mg mTHPCkg$+$MIN@1$/ iv. Each two animals were perfused withnormal saline and Zamboni fixative 6, 12, 24, 48 and 72hours after photosensitization. Untreated animalsserved as controls. Fluorescence was quantified on 20micrometer frozen sections with CCD-camera andappropriate software. Immunohistochemistry identifiedspecific cell types with antibodies tosteroid-synthesizing enzymes. The cortex exhibited anintense fluorescence, with weaker fluorescence ofcorticocytes in the zona glomerulosa compared to theother zones. Besides intensely fluorescing singly lyingscattered cells, the medulla showed a faintmTHPC-induced fluorescence. Immunohistochemistryrevealed that intramedullary cells with intensefluorescence were corticocytes, showing a positivereaction to the 21-$beta@-hydroxylase antibody. Peakaccumulation of mTHPC was always observed after 24hours. Our results indicate for the first time thatonly steroid synthesizing cells of the adrenal glandexhibit an intense photosensitizer-inducedfluorescence. Thus mTHPC-application is anuncomplicated method to identify steroid-synthesizingcells, possibly also in other organs. !15
机译：摘要：全身施用中四（羟苯基）二氢卟酚（mTHPC）后，大鼠肾上腺发出强烈的荧光。我们调查了肾上腺的哪些部位积聚了mTHPC。此外，我们检查了肾上腺mTHPC积累的时间过程。十只雄性Wistar大鼠每只被给予0.5或0.7mg mTHPCkg ++ MIN @ 1 $ / iv。在光敏化后的6、12、24、48和72小时，每两只动物被灌注生理盐水和Zamboni固定剂。未经处理的动物作为对照。用CCD相机和适当的软件在20微米冷冻切片上定量荧光。免疫组织化学用类固醇合成酶抗体鉴定了特定的细胞类型。皮质表现出强烈的荧光，与其他区域相比，肾小球透明带中的皮质细胞荧光弱。除了强烈发散单个躺散的细胞的荧光外，延髓还显示出淡淡的THPC诱导的荧光。免疫组织化学显示，具有强烈荧光的髓内细胞是皮质细胞，对21-β-羟化酶抗体呈阳性反应。总是在24小时后观察到mTHPC的峰值积累。我们的结果首次表明只有肾上腺的类固醇合成细胞表现出强烈的光敏剂诱导的荧光。因此，mTHPC的应用是鉴定类固醇合成细胞（可能也在其他器官中）的简单方法。 ！15

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