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首页> 外文会议>Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II >Photodynamic therapy-induced programmed cell death in carcinoma cell lines
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Photodynamic therapy-induced programmed cell death in carcinoma cell lines

机译：光动力疗法诱导癌细胞系程序性细胞死亡

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Abstract: The mode of cell death following photodynamic therapy (PDT) was investigated from the perspective of programmed cell death (apoptosis). Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a), and rat mammary carcinoma (MTF7) were treated by PDT following sensitization with dihematoporphyrin ether (DHE). The response of these carcinoma cell lines to PDT was variable. An examination of extracted cellular DNA by gel electrophoresis showed the characteristic DNA ladder pattern indicative of internucleosomal cleavage of DNA during apoptosis. MTF7 and PC3 responded to PDT by inducing apoptosis while H322a had no apoptotic response. The magnitude of the response and the PDT dosage required to induce the effect were different in PC3 and MTF7. MTF7 cells responded with rapid apoptosis at the dose of light and drug that yielded 50% cell death (LD$-50$/). In contrast, PC3 showed only marginal apoptosis at the LD$-50$/ but had a marked response at the LD$-85$/. Furthermore, the onset of apoptosis followed slower kinetics in PC3 (2 hr - 4 hr) than in MTF7 ($LS 1 hr). H322a cells were killed by PDT but failed to exhibit any apoptotic response. This study indicates that apoptosis may occur during PDT induced cell death, but this pathway is not universal for all cancer cell lines.!33
机译：摘要：从程序性细胞死亡（细胞凋亡）的角度研究了光动力疗法（PDT）后的细胞死亡方式。在用二血卟啉醚（DHE）致敏后，PDT处理人前列腺癌细胞（PC3），人非小细胞肺癌（H322a）和大鼠乳腺癌（MTF7）。这些癌细胞系对PDT的反应是可变的。通过凝胶电泳检查提取的细胞DNA显示出特征性的DNA梯形图样，表明细胞凋亡期间DNA的核小体切割。 MTF7和PC3通过诱导细胞凋亡来响应PDT，而H322a没有凋亡反应。在PC3和MTF7中，诱导效果所需的反应强度和PDT剂量不同。 MTF7细胞在光和药物剂量下迅速凋亡，产生50％的细胞死亡（LD $ -50 $ /）。相比之下，PC3在LD $ -50 $ /处仅显示边缘凋亡，而在LD $ -85 $ /处有明显的反应。此外，PC3（2 hr-4 hr）中的凋亡发生跟随动力学较MTF7（$ LS 1 hr）慢。 H322a细胞被PDT杀死，但未表现出任何凋亡反应。这项研究表明，细胞凋亡可能在PDT诱导的细胞死亡期间发生，但该途径并非在所有癌细胞系中都通用！33

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