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Measuring stall forces in vivo with optical tweezers through light momentum changes

机译:通过光镊改变光镊测量体内失速力

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摘要

The stall forces of processive molecular motors have been widely studied previously in vitro. Even so, in vivo experiments are required for determining the actual performance of each molecular motor in its natural environment. We report the direct measurement of light momentum changes in single beam optical tweezers as a suitable technique for measuring forces inside living cells, where few alternatives exist. The simplicity of this method, which does not require force calibration for each trapped object, makes it convenient for measuring the forces involved in fast dynamic biological processes such us intracellular traffic. Here we present some measurements of the stall force of processive molecular motors inside living Allium cepa cells.
机译:以前在体外已经广泛研究了过程分子电动机的失速力。即使这样,仍需要进行体内实验来确定每种分子马达在其自然环境中的实际性能。我们报告了直接测量单束光镊中光动量变化的方法,这是一种用于测量活细胞内部力的合适技术,其中几乎没有其他选择。该方法的简单性,不需要对每个被困物体进行力校准,因此方便了测量快速动态生物过程(例如细胞内运输)中涉及的力。在这里,我们介绍一些在活的洋葱cepa细胞内部的分子分子的失速力的测量值。

著录项

  • 来源
    《Optical trapping and optical micromanipulation VIII》|2011年|p.809726.1-809726.10|共10页
  • 会议地点 San Diego CA(US)
  • 作者单位

    Optical Trapping Lab - Grup de Biofotonica, Departament de Fisica Aplicada i Optica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08028, Spain;

    Optical Trapping Lab - Grup de Biofotonica, Departament de Fisica Aplicada i Optica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08028, Spain;

    Optical Trapping Lab - Grup de Biofotonica, Departament de Fisica Aplicada i Optica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08028, Spain;

    Optical Trapping Lab - Grup de Biofotonica, Departament de Fisica Aplicada i Optica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08028, Spain;

    Optical Trapping Lab - Grup de Biofotonica, Departament de Fisica Aplicada i Optica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08028, Spain;

    Optical Trapping Lab - Grup de Biofotonica, Departament de Fisica Aplicada i Optica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08028, Spain;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 光学;
  • 关键词

    optical tweezers; force measurements; light momentum; molecular motors; in vivo; stall force; myosin;

    机译:光学镊子;力测量;轻的动量;分子马达体内;失速力肌球蛋白;

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