• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Optical trapping and microscopic imaging >Multifocus CARS microscopy for realtime vibrational imaging
【24h】

Multifocus CARS microscopy for realtime vibrational imaging

机译：多焦点汽车显微镜用于实时振动成像

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We developed a multifocus excitation coherent anti-Stokes Raman scattering microscope using a microlens array scanner for realtime molecular imaging. Two picoseond mode-locked lasers tightly synchronized were splited to a few tens of foci with the microlens array, the foci excited the sample parallely and the generated CARS from each spot was detected with an image sensor at once. By the multifocus excitation, exposure time was prolonged proportionally to the number of the foci because of parallel excitation and detection. The video-rate (frame rate of 30 fps) imaging of polystyrene beads in water was demonstrated, and the Brownian motion of beads were clearly obtained. The three-dimensional reconstructed imaging of living HeLa cells (frame rate of 5 fps, 85 images) was also demonstrated.
机译：我们开发了一种使用微透镜阵列扫描仪进行实时分子成像的多焦点激发相干反斯托克斯拉曼散射显微镜。用微透镜阵列将两个紧密同步的皮秒级锁模激光器分成几十个焦点，该焦点平行激发样品，并立即用图像传感器检测每个点产生的CARS。通过多焦点激发，由于平行激发和检测，曝光时间与焦点的数量成比例地延长。演示了聚苯乙烯珠在水中的视频速率（帧速率为30 fps）成像，并清楚地获得了珠的布朗运动。还展示了活HeLa细胞的三维重建成像（帧频为5 fps，85张图像）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号