Quantitative phase microscopy of red blood cells with slightly-off-axis interference

机译：血红细胞定量相显微镜检测，偏轴干扰轻微

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Microscopic interferometry is a noncontact technique for quantitative phase imaging of live cells. The method combines the principles of single-shot slightly-off-axis interferometry and confocal microscopy and is characterized by real-time acquisition capabilities and optimized spatial resolution. However, slightly-off-axis interferometry requires less detector bandwidth than traditional off-axis interferometry and fewer phase-shifted steps than on-axis interferometry. Meanwhile, confocal microscopy allows microstructure magnification imaging. To validate the utility of this technique, experimental and theoretical comparisons are given. The potential of the technique for phase microcopy is demonstrated by experiments on red blood cells. This study will set the basis for interferometric phase measurements of dynamic processes with fine spatial details, especially for observing live biological cell dynamics.

机译：显微干涉术是用于活细胞定量相位成像的非接触技术。该方法结合了单次微偏轴干涉测量和共聚焦显微镜的原理，并具有实时采集功能和优化的空间分辨率。然而，与传统的离轴干涉测量法相比，稍微偏轴的干涉测量法需要更少的检测器带宽，并且比同轴的干涉测量法需要更少的相移步长。同时，共聚焦显微镜允许显微组织放大成像。为了验证该技术的实用性，给出了实验和理论上的比较。通过对红细胞的实验证明了相显微镜技术的潜力。这项研究将为具有精细空间细节的动态过程的干涉相测量提供基础，尤其是观察活生物细胞动力学。

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来源
《Optics in health care and biomedical optics IV》|2010年|p.784505.1-784505.8|共8页
会议地点 Beijing(CN)
作者
Xue Liang; Lai Jian-cheng; Li Zhen-hua;
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作者单位

Department of Information Physics and Engineering, Nanjing University of Science andrnTechnology, Nanjing 210094, China;

Department of Information Physics and Engineering, Nanjing University of Science andrnTechnology, Nanjing 210094, China;

rnDepartment of Information Physics and Engineering, Nanjing University of Science andrnTechnology, Nanjing 210094, China;

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原文格式 PDF
正文语种 eng
中图分类光、激光生物医学;
关键词
Medical optics; Quantitative phase distribution; Slightly-off-axis interferometry; Red blood cell; Confocal microscopy;

机译：医疗光学；定量相分布；偏轴干涉法；红细胞;共聚焦显微镜;
入库时间 2022-08-26 13:44:48

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1. Quantitative phase imaging of human red blood cells using phase-shifting white light interference microscopy with colour fringe analysis [J] . Singh Mehta Dalip, Srivastava Vishal Applied Physics Letters . 2012,第20期

机译：使用相移白光干涉显微镜和色带分析对人红细胞进行定量相成像
2. Quantitative phase imaging of human red blood cells using phase-shifting white light interference microscopy with colour fringe analysis [J] . Dalip Singh Mehta, Vishal Srivastava Applied Physics Letters . 2012,第20期

机译：使用相移白光干涉显微镜和色带分析对人红细胞进行定量相成像
3. Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy [J] . Norikazu Sugiyama, Yasuyuki Asai, Toyohiko Yamauchi, Biomedical Optics Express . 2012,第9期

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4. Cellular phase observations and measurements on red blood cells affected by lithium and lead ions with quantitative interferometric microscopy [C] . Qi Wei, Zhilong Jiang, Yan Kong, Optics in health care and biomedical optics VIII . 2018

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5. Exploring Different Textures of Nematic Liquid Crystal Cells for Quantitative Fourier Phase Contrast Microscopy [D] . Kyeremah, Charlotte. 2020

机译：用于定量傅里叶相腺逆相显微镜的探索甲型液晶细胞的不同纹理
6. Single-shot slightly-off-axis interferometry based Hilbert phase microscopy of red blood cells [O] . Liang Xue, Jiancheng Lai, Shouyu Wang, 2011

机译：基于单脉冲微偏轴干涉法的红细胞希尔伯特相显微镜
7. Single-shot slightly-off-axis interferometry based Hilbert phase microscopy of red blood cells [O] . Xue, Liang, Lai, Jiancheng, Wang, Shouyu, 2011

机译：基于单脉冲微偏轴干涉法的红细胞希尔伯特相显微镜

Quantitative phase microscopy of red blood cells with slightly-off-axis interference

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