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首页> 外文会议>Optics in health care and biomedical optics VI >In vitro sensitivity of Candida spp. to hematoporphyrin monomethyl ether-mediated photodynamic inactivation
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In vitro sensitivity of Candida spp. to hematoporphyrin monomethyl ether-mediated photodynamic inactivation

机译：念珠菌的体外敏感性。血卟啉单甲醚介导的光动力学失活

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摘要

Background: An increasing prevalence of Candida infections has emerged with the wide use of immune-suppressants and antibiotics. Current antifungal drugs exhibit low efficiency and high toxicity to the normal organs. Photodynamic inactivation (PDI) provides an alternative therapeutic strategy involving the use of photosensitizer (PS) and light irradiation. This study evaluated PDI effects against strains of C. albicans, C. parapsilosis, C. krusei and C. glabrata, using the PS of hematoporphyrin monomethyl ether (HMME), which is a second-generation PS clinically approved in China. Methods: Suspensions (～10~6 CFU/ml) were incubated with seven HMME concentrations (0.25～50 μM) for 30 min followed by 532-nm laser irradiation for 10 min at 40 mW/cm~2. Viability of cells was assayed by CFU counting. Furthermore, fetal calf serum (10%) and singlet oxygen quencher sodium azide (100mM) were respectively added to the suspension of C. krusei to evaluate their roles in PDI process. Results: Among the four species, C. albicans was the most sensitive to PDI; 4 log_(10) killing was achieved at the concentration of 7.5 μM. C. glabrata was the most resistant; 3 log_(10) killing was not obtained even at PS concentration of 50 μM. PDI effects against C. krusei were inhibited by both serum and sodium azide. Conclusions: HMME-mediated PDI was able to effectively kill Candida in our experimental conditions, mainly through a Type Ⅱ photoprocess. However, the effects could be intensively reversed by the presence of serum. Thus, there might be a long way before HMME can be used in fighting against Candida in real infectious foci.
机译：背景：随着免疫抑制剂和抗生素的广泛使用，念珠菌感染的患病率正在上升。当前的抗真菌药物对正常器官表现出低效率和高毒性。光动力灭活（PDI）提供了另一种治疗策略，涉及使用光敏剂（PS）和光照射。本研究使用血卟啉单甲醚（HMME）的PS评估了PDI对白色念珠菌，副念珠菌，克鲁斯梭菌和光滑念珠菌菌株的PDI作用，这是中国在临床上批准的第二代PS。方法：将悬浮液（〜10〜6 CFU / ml）与七种HMME浓度（0.25〜50μM）孵育30分钟，然后以532 nm激光以40 mW / cm〜2辐照10分钟。通过CFU计数测定细胞的活力。此外，将胎牛血清（10％）和单线态氧淬灭剂叠氮化钠（100mM）分别添加到克鲁氏梭菌的悬浮液中，以评估它们在PDI过程中的作用。结果：在这四个物种中，白色念珠菌对PDI最敏感。在7.5μM的浓度下可杀死4 log_（10）。光滑念珠菌最耐；即使在50μM的PS浓度下也未获得3 log_（10）杀灭。血清和叠氮化钠均抑制了对克鲁氏梭菌的PDI作用。结论：在我们的实验条件下，HMME介导的PDI能够有效杀死念珠菌，主要是通过Ⅱ型光致过程。但是，血清的存在可以强烈逆转这种作用。因此，在真正的传染病灶中使用HMME对抗念珠菌可能还有很长的路要走。

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  • 来源
    《Optics in health care and biomedical optics VI》|2014年|92680L.1-92680L.7|共7页
  • 会议地点 Beijing(CN)
  • 作者

    Yucheng Wang; Ying Wang; Sumin Wu; Ying Gu;

  • 作者单位

    Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China,College of Medicine, Nankai University, Tianjin 300071, China;

    Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China;

    Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China;

    Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Candida; Photodynamic inactivation; HMME;

    机译：念珠菌光动力失活； HMME;

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