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首页> 外文会议>Optics in health care and biomedical optics VI >A photoelastic modulator-based birefringence imaging microscope for measuring biological specimens
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A photoelastic modulator-based birefringence imaging microscope for measuring biological specimens

机译：基于光弹性调制器的双折射成像显微镜，用于测量生物样本

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摘要

The photoelastic modulator (PEM) has been applied to a variety of polarimetric measurements. However, nearly all such applications use point-measurements where each point (spot) on the sample is measured one at a time. The main challenge for employing the PEM in a camera-based imaging instrument is that the PEM modulates too fast for typical cameras. The PEM modulates at tens of KHz. To capture the specific polarization information that is carried on the modulation frequency of the PEM, the camera needs to be at least ten times faster. However, the typical frame rates of common cameras are only in the tens or hundreds frames per second. In this paper, we report a PEM-camera birefringence imaging microscope. We use the so-called stroboscopic illumination method to overcome the incompatibility of the high frequency of the PEM to the relatively slow frame rate of a camera. We trigger the LED light source using a field-programmable gate array (FPGA) in synchrony with the modulation of the PEM. We show the measurement results of several standard birefringent samples as a part of the instrument calibration. Furthermore, we show results observed in two birefringent biological specimens, a human skin tissue that contains collagen and a slice of mouse brain that contains bundles of myelinated axonal fibers. Novel applications of this PEM-based birefringence imaging microscope to both research communities and industrial applications are being tested.
机译：光弹性调制器（PEM）已应用于各种极化测量。但是，几乎所有此类应用程序都使用点测量，一次测量一个样本上的每个点（点）。在基于摄像机的成像仪器中使用PEM的主要挑战是，对于典型的摄像机，PEM的调制速度太快。 PEM以数十KHz的频率进行调制。为了捕获在PEM的调制频率上承载的特定偏振信息，相机需要至少快十倍。但是，普通摄像机的典型帧速率仅为每秒数十或数百帧。在本文中，我们报告了P​​EM相机双折射成像显微镜。我们使用所谓的频闪照明方法来克服PEM的高频与照相机相对较慢的帧频之间的不兼容性。我们使用现场可编程门阵列（FPGA）与PEM的调制同步触发LED光源。作为仪器校准的一部分，我们显示了几种标准双折射样品的测量结果。此外，我们显示了在两个双折射生物学标本中观察到的结果，一个是包含胶原蛋白的人皮肤组织，另一个是包含髓鞘轴突纤维束的小鼠大脑切片。这种基于PEM的双折射成像显微镜在研究社区和工业应用中的新应用正在测试中。

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