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首页> 外文会议>Oral-Based Diagnostics; Annals of the New York Academy of Sciences; vol.1098 >Lab-on-a-Chip Methods for Point-of-Care Measurements of Salivary Biomarkers of Periodontitis
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Lab-on-a-Chip Methods for Point-of-Care Measurements of Salivary Biomarkers of Periodontitis

机译：牙周炎唾液生物标志物即时护理的芯片实验室方法

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摘要

Salivary secretions contain a variety of molecules that reflect important pathophysiological activities. Quantitative changes of specific salivary biomarkers could have significance in the diagnosis and management of both oral and systemic diseases. Modern point-of-care technologies with enhanced detection capabilities are needed to implement a significant advancement in salivary diagnostics. One such promising technology is the recently described lab-on-a-chip (LOC) assay system, in which assays are performed on chemically sensitized beads populated into etched silicon wafers with embedded fluid handling and optical detection capabilities. Using this LOC system, complex assays can be performed with small sample volumes, short analysis times, and markedly reduced reagent costs. This report describes the use of LOC methodologies to assess the levels of interleukin-1β (IL-1β), C-reactive protein (CRP), and matrix metalloproteinase-8 (MMP-8) in whole saliva, and the potential use of these biomarkers for diagnosing and categorizing the severity and extent of periodontitis. This study demonstrates that the results achieved by the LOC approach are in agreement with those acquired with standard enzyme-linked immunosorbent assay (ELISA), with significant IL-1β and MMP-8 elevations in whole saliva of periodontitis patients. Furthermore, because of the superior detection capacities associated with the LOC approach, unlike those with ELISA, significant differences in CRP levels between periodontitis patients and normal subjects are observed. Finally, principal component analysis (PCA) is performed to yield an efficient method to discriminate between peri-odontally healthy and unhealthy patients, thus increasing the diagnostic value of these biomarkers for periodontitis when examined with the integrated LOC sensor system.
机译：唾液分泌物包含多种反映​​重要病理生理活动的分子。特定唾液生物标志物的定量变化可能对口腔和全身疾病的诊断和管理具有重要意义。为了实现唾液诊断的重大进步，需要具有增强检测能力的现代即时医疗技术。一种这样的有前途的技术是最近描述的芯片实验室（LOC）分析系统，该系统对具有嵌入的液体处理和光学检测功能的化学致敏珠进行检测，这些珠被植入蚀刻的硅片中。使用该LOC系统，可以以小样品量，短分析时间和显着降低的试剂成本进行复杂的测定。本报告介绍了使用LOC方法评估整个唾液中白介素1β（IL-1β），C反应蛋白（CRP）和基质金属蛋白酶8（MMP-8）的水平，以及这些方法的潜在用途用于诊断和分类牙周炎的严重程度和程度的生物标志物。这项研究表明，通过LOC方法获得的结果与通过标准酶联免疫吸附测定（ELISA）获得的结果一致，在牙周炎患者的整个唾液中IL-1β和MMP-8明显升高。此外，由于与LOC方法相关的优越检测能力，与ELISA方法不同，在牙周炎患者与正常受试者之间CRP水平存在显着差异。最后，进行主成分分析（PCA）以产生一种区分牙周健康和不健康患者的有效方法，从而在使用集成式LOC传感器系统进行检查时增加了这些生物标记物对牙周炎的诊断价值。

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