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Longitudinal in vivo transcutaneous observation of Raman signals from breast cancer during chemotherapy in small animal model

机译:小动物模型化疗期间乳腺癌拉曼信号的纵向体内经皮观察

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摘要

Because mammography, the gold standard of breast cancer screening and monitoring treatment efficacy, has limitations, there is a necessity to have a new method for breast cancer patients. Raman spectroscopy is considered as one of the best alternative approaches due to its ability of visualizing (bio)chemical information of a matter. In this study, we hypothesized that the change of biochemical composition occurs earlier than morphological change in breast cancer during chemotherapy, and attempted to prove it by employing fiber-optic Raman spectroscopy for longitudinal Raman measurement in small animal breast cancer model. To confirm the hypothesis, we measured Raman spectra of a tumor breast and the contralateral breast during chemotherapy for 4 fisher 344 female rats longitudinally. Principal component analysis and Raman spectral differences between breast tumor and contralateral normal breast did not show a clear difference between them which may have been caused by interference from skin. Thus, spatially-offset Raman spectroscopy will be employed in order to acquire the Raman signal directly from tumor while suppressing Raman signal from skin for the future study.
机译:由于乳房X线照相术是乳腺癌筛查和监测治疗效果的金标准,因此存在局限性,因此有必要为乳腺癌患者提供一种新的方法。由于拉曼光谱能够可视化物质的(生物)化学信息,因此被认为是最佳的替代方法之一。在这项研究中,我们假设化学过程中乳腺癌发生过程中化学成分的变化要早于形态学变化,并试图通过光纤拉曼光谱法在小动物乳腺癌模型中通过纵向拉曼测量来证明这一点。为了证实这一假设,我们纵向测量了4只Fisher 344雌性大鼠化疗期间肿瘤乳房和对侧乳房的拉曼光谱。乳腺肿瘤和对侧正常乳腺之间的主成分分析和拉曼光谱差异未显示出它们之间的明显差异,这可能是由于皮肤干扰所致。因此,为了进一步从肿瘤中获取拉曼信号,同时抑制来自皮肤的拉曼信号,将采用空间偏移拉曼光谱法。

著录项

  • 来源
    《Photonic therapeutics and diagnostics XI》|2015年|93032U.1-93032U.5|共5页
  • 会议地点 San Francisco CA(US)
  • 作者单位

    Department of Medical System Engineering Gwangju Institute of Science and Technology (GIST), 123 Cheomdan Gwagiro, Bukgu, Gwangju, South Korea;

    Advanced Photonics Research Institute Gwangju Institute of Science and Technology (GIST), 123 Cheomdan Gwagiro, Bukgu, Gwangju, South Korea;

    Advanced Photonics Research Institute Gwangju Institute of Science and Technology (GIST), 123 Cheomdan Gwagiro, Bukgu, Gwangju, South Korea;

    Department of Medical System Engineering Gwangju Institute of Science and Technology (GIST), 123 Cheomdan Gwagiro, Bukgu, Gwangju, South Korea,School of Information and Communications Gwangju Institute of Science and Technology (GIST), 123 Cheomdan Gwagiro, Bukgu, Gwangju, South Korea;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Breast cancer; Chemotherapy; Raman spectroscopy (RS); Principal component analysis (PCA); Longitudinal study; Cyclophosphamide (CTX);

    机译:乳腺癌;化学疗法拉曼光谱(RS);主成分分析(PCA);纵向研究;环磷酰胺(CTX);

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