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The use of coconut fiber and antagonistic Pseudomonas CMR12a for biological control of Rhizoctonia damping-off on Chinese cabbage (Brassica rapa)

机译:椰子纤维和拮抗假单胞菌CMR12a抑制大白菜对根瘤菌的抑菌作用

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Isolated from cocoyam roots in Cameroon, Pseudomonas CMR12a has been explored as a promising antagonistic agent due to its capacity to produce phenazines and biosurfactants. CMR12a and mutants of this strain impaired in phenazine and/or biosurfactant production, were applied at a concentration of 107 CFU g﹣1 soil to control damping-off caused by Rhizoctonia solani AG 2-1 on Chinese cabbage. Data recorded 14 days after inoculation indicated that disease severity had decreased from 3.5 to 1.42 in CMR12a-treated seedlings. In soil inoculated with phenazine- or biosurfactant-deficient mutants, seedlings were partially protected and disease score was 2.29 and 1.96, respectively. However, the disease suppressive effect was completely lost in soil inoculated with a mutant impaired in the production of both compounds. These data suggest that both phenazines and biosurfactants were responsible for the profound effects of Pseudomonas CMR 12a.
机译:假单胞菌CMR12a是从喀麦隆的可可粉根中分离出来的,因为它具有生产吩嗪和生物表面活性剂的能力,因此被认为是一种有前途的拮抗剂。以107 CFU g and1的土壤浓度施用CMR12a和该菌株的吩嗪和/或生物表面活性剂生产受损的突变体,以控制大白菜上的茄红枯菌2-1引起的阻尼。接种14天后记录的数据表明,经CMR12a处理的幼苗的病害严重程度已从3.5降低至1.42。在用吩嗪或生物表面活性剂缺陷型突变株接种的土壤中,幼苗受到了部分保护,病害评分分别为2.29和1.96。但是,在两种化合物生产中受损的突变体接种的土壤中,疾病抑制作用完全丧失。这些数据表明吩嗪和生物表面活性剂均对假单胞菌CMR 12a的深远影响负责。

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