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Biological Decontamination Using Pulsed Filamentary Microplasma Jet

机译：脉冲丝状微等离子体射流的生物净化

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摘要

Microplasma jet for the generation of pulsed filamentary discharge at atmospheric pressure has been devised for biological decontamination as well as for modification of surface properties. Long plasma-filament is generated inside a quartz tube and characterized using optical emission spectroscopy, current voltage measurements, numerical simulations and microphotography. Efficiency of our plasma source for the decontamination on inner surface of the tube as well as on objects placed in proximity of plasma effluent is studied. Escherichia coli (Gram-negative bacteria) and spores of Bacillus atrophaeus (Gram-positive bacteria) are used for the decontamination studies. Decontamination of Bacillus atrophaeus endospores, which are layered on PET polymer material, and placed in the proximity of plasma effluent, shows the mean logarithmic bacterial reduction of 3.67 for the treatment time of 120 s. Inactivation of Escherichia coli coated on inner surface of the tube shows the mean logarithmic bacterial reduction of about 5 for the treatment time of 30 s. In addition to this, inhibition studies of bacteria coated on agar plate are also carried out. It shows plasma effluent generated in our plasma source is very effective for the inhibition of bacterial colonization.
机译：已经设计出用于在大气压下产生脉冲丝状放电的微等离子体射流，以用于生物净化以及改变表面性能。石英管内部会产生长的等离子丝，并使用光发射光谱法，电流电压测量，数值模拟和显微摄影对其进行表征。研究了我们的等离子体源对管内表面以及放置在等离子体流出物附近的物体进行净化的效率。大肠杆菌（革兰氏阴性细菌）和萎缩芽孢杆菌的孢子（革兰氏阳性细菌）用于去污研究。萎缩芽孢杆菌内生孢子的去污作用位于PET聚合物材料上，并置于血浆流出物附近，在120 s的处理时间内，平均对数细菌减少量为3.67。包被在试管内表面的大肠杆菌的灭活显示，在30 s的处理时间内，平均对数细菌减少了约5。除此之外，还进行了对涂在琼脂平板上的细菌的抑制研究。它表明在我们的血浆源中产生的血浆流出物对于抑制细菌定植非常有效。

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