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首页> 外文会议>Plasma for bio-decontamination, medicine and food security >Investigations of Bacterial Inactivation and DNA Fragmentation Induced by Flowing Humid Argon Post-discharge
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Investigations of Bacterial Inactivation and DNA Fragmentation Induced by Flowing Humid Argon Post-discharge

机译：流动的氩气后放电引起的细菌失活和DNA片段化的研究

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摘要

Bio-contaminated surfaces were exposed to an atmospheric pressure flowing post-discharge, i.e. without direct contact of the plasma with the surface. The non-thermal plasma source was a dielectric barrier discharge. Using humid argon as a feed gas, a reduction of six orders of magnitude of survivors could be obtained for Escherichia coli. An investigation of bacterial inactivation mechanisms during the plasma induced treatment was conducted. For this purpose, DNA (plas-mid and genomic DNA in aqueous solution) degradation by the plasma process was studied, assuming that the bacterial inactivation is obtained when the bacterial DNA is fragmented. According to the operating conditions (feed gas, reactor geometry and discharge input power), DNA fragmentation was evaluated in correlation with aqueous phase hydrogen peroxide concentration measurements. It appears that hydrogen peroxide is not the only factor responsible for DNA fragmentation and that short-lived species produced by water dissociation are major contributors.
机译：生物污染的表面在放电后暴露于大气压下流动，即等离子体与表面不直接接触。非热等离子体源是介电势垒放电。使用潮湿的氩气作为进料气，大肠杆菌可以减少六个数量级的幸存者。进行了等离子体诱导处理过程中细菌失活机制的研究。为此目的，研究了通过血浆过程降解DNA（水溶液中的质粒质粒和基因组DNA）的过程，假设当细菌DNA片段化时会导致细菌失活。根据操作条件（进料气，反应器的几何形状和排出的输入功率），与水相过氧化氢浓度测量值相关地评估了DNA片段化。似乎过氧化氢不是造成DNA片段化的唯一因素，而水解离产生的短命物种是主要的贡献者。

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  • 会议地点 Demanovska Dolina(SK)
  • 作者

    Emmanuel Odic; S. Limam; M.J. Kirkpatrick; B. Dodet; S. Salamitou; M.S. DuBow;

  • 作者单位

    E3S - Department of Power and Energy Systems, SUPELEC, 3, rue Joliot-Curie, F-91192 Gif-sur-Yvette Cedex, France;

    E3S - Department of Power and Energy Systems, SUPELEC, 3, rue Joliot-Curie, F-91192 Gif-sur-Yvette Cedex, France;

    E3S - Department of Power and Energy Systems, SUPELEC, 3, rue Joliot-Curie, F-91192 Gif-sur-Yvette Cedex, France;

    E3S - Department of Power and Energy Systems, SUPELEC, 3, rue Joliot-Curie, F-91192 Gif-sur-Yvette Cedex, France;

    Institut de Genetique et Microbiologie, Universite Paris-Sud 11, F-91405 Orsay, France;

    Institut de Genetique et Microbiologie, Universite Paris-Sud 11, F-91405 Orsay, France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物工程学（生物技术）;
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