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首页> 外文会议>Plasmonics: Metallic nanostructures and their optical properties VIII >SURFACE PLASMON-ENHANCED AND QUENCHED TWO-PHOTONEXCITED FLUORESCENCE
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SURFACE PLASMON-ENHANCED AND QUENCHED TWO-PHOTONEXCITED FLUORESCENCE

机译：表面等离激元增强和猝灭的两光子 r n激发的荧光

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摘要

This study investigated theoretically and experimentally that two-photon excited fluorescence is enhanced and quenched via surface plasmons (SPs) excited by total internal reflection with a silver film. The fluorescence intensity is fundamentally affected by the local electromagnetic field enhancement and the quantum yield change according to the surrounding structure and materials. By utilizing the Fresnel equation and classical dipole radiation modeling, local electric field enhancement, fluorescence quantum yield, and fluorescence emission coupling yield via SPs were theoretically analyzed at different dielectric spacer thicknesses between the fluorescence dye and the metal film. The fluorescence lifetime was also decreased substantially via the quenching effect. A two-photon excited total internal reflection fluorescence (TIRF) microscopy with a time-correlated single photon counting device has been developed to measure the fluorescence lifetimes, photostabilities, and enhancements. The experimental results demonstrate that the fluorescence lifetimes and the trend of the enhancements are consistent with the theoretical analysis. The maximum fluorescence enhancement factor in the surface plasmon-total internal reflection fluorescence (SP-TIRF) configuration can be increased up to 30 fold with a suitable thickness SiO_2 spacer. Also, to compromise for the fluorescence enhancement and the fluorophore photostability, we find that the SP-TIRF configuration with a 10 nm SiO_2 spacer can provide an enhanced and less photobleached fluorescent signal via the assistance of enhanced local electromagnetic field and quenched fluorescence lifetime, respectively.
机译：这项研究在理论上和实验上都研究了通过由银膜全内反射激发的表面等离激元（SP）来增强和消除双光子激发的荧光。荧光强度基本上受局部电磁场增强和量子产率随周围结构和材料的变化的影响。通过使用菲涅耳方程和经典偶极子辐射模型，理论上分析了荧光染料与金属膜之间不同介电间隔物厚度下的局部电场增强，荧光量子产率和通过SP的荧光发射耦合产率。荧光寿命也通过猝灭作用而显着降低。具有时间相关的单光子计数装置的双光子激发全内反射荧光（TIRF）显微镜已经开发出来，可以测量荧光寿命，光稳定性和增强作用。实验结果表明，荧光寿命和增强趋势与理论分析是一致的。使用合适厚度的SiO_2隔离层，可以将表面等离振子-全内反射荧光（SP-TIRF）配置中的最大荧光增强因子提高至30倍。同样，为了兼顾荧光增强和荧光团的光稳定性，我们发现带有10 nm SiO_2间隔基的SP-TIRF配置可以分别通过增强的局部电磁场和淬灭的荧光寿命来提供增强的且光漂白较少的荧光信号。 。

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  • 来源
  • 会议地点 San Diego CA(US)
  • 作者

    C.-Y. Lin; C.-H. Lien; K.-C. Chiu; C.-Y. Chang; S.-H. Chang; T.-F. Guo; S.-J. Chen;

  • 作者单位

    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan;

    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan;

    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan;

    Institute of Electro-Optical Science and Engineering, National Cheng Kung University, Tainan 701,rnTaiwan;

    Institute of Electro-Optical Science and Engineering, National Cheng Kung University, Tainan 701,rnTaiwan;

    Institute of Electro-Optical Science and Engineering, National Cheng Kung University, Tainan 701,rnTaiwan;

    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701,rnTaiwan;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 金属材料;
  • 关键词

    surface plasmon; two-photon excited fluorescence; fluorescence lifetime; quantum yield;

    机译：表面等离激元双光子激发荧光；荧光寿命量子产率;

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