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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Cultivated Edible Mushrooms Pleurotus pulmonarius and P. sajor-caju are Two Separate Species as Established by Molecular and Crossing Analyses
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Cultivated Edible Mushrooms Pleurotus pulmonarius and P. sajor-caju are Two Separate Species as Established by Molecular and Crossing Analyses

机译：栽培食用菌侧耳和P. sajor-caju是通过分子和杂交分析建立的两个独立物种

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摘要

Data on morphological and molecular characterization of the two cultivated oyster mushroom species, P. sajor-caju and P. pulmonarius are presented. These species are very similar in morphology and substrate specificity. However, species identity for P. sajor-caju is still under discussion. Some researchers believe P. sajor-caju is a synonym of the species P. pulmonarius while the others consider P. sajor-caju as a separate species. The purpose of the research was to solve the intricacy of P. sajorcaju species. We employed two approaches in the analysis-molecular and conventional crossing. Molecular analysis of the variable ITS and IGS regions of the rRNA genes cluster did not allow to differentiate reliably between morphologically similar species P. pulmonarius and P. sajor-caju as well as to confirm species identity for P. sajor-caju strains CS-32, H-1, H-2. Sexual (mating) compatibility between haploid tester strains of these two species as performed in mon-mon crossing experiments demonstrated complete reproductive isolation between P. pulmonarius and P. sajor-caju species and, therefore, their separate species identity.
机译：介绍了两种栽培的牡蛎蘑菇物种P. sajor-caju和P. pulmonarius的形态和分子表征数据。这些物种的形态和底物特异性非常相似。然而，关于P. sajor-caju的物种身份仍在讨论中。一些研究人员认为，P。sajor-caju是P. pulmonarius物种的同义词，而其他研究人员则认为P. sajor-caju是单独的物种。该研究的目的是解决P. sajorcaju物种的复杂性。我们在分析中采用了两种方法-分子杂交和常规杂交。对rRNA基因簇的可变ITS和IGS区域进行分子分析，无法可靠地区分形态相似的P. pulmonarius物种和P. sajor-caju物种，也无法确认P. sajor-caju菌株CS-32的物种同一性，H-1，H-2。在mon-mon杂交实验中，这两个物种的单倍体测试菌株之间的性（交配）相容性表明，P。pulmonarius和P. sajor-caju物种之间完全生殖隔离，因此它们具有独立的物种同一性。

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