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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Detection of Trichoderma aggressivum Green Mould During Spawn-run
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Detection of Trichoderma aggressivum Green Mould During Spawn-run

机译：产卵期木霉绿色霉菌的检测

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Since 2006, green mould (Trichoderma aggressivum) has presented big problems to the Dutch mushroom industry. T. aggressivum infects compost at a very early stage and in the Dutch situation infection most likely takes place at the compost yard. Even though compost producers in the Netherlands are very keen to prevent green mould problems, occasionally still a number of crops get infected. Therefore there is a need for a method that allows early detection of Trichoderma green mould. Agaricus bisporus and T. aggressivum use volatiles to affect each other's growth rate. We tested the possibility of detecting Trichoderma green mould using the volatiles that are produced during spawn run. We used an untargeted metabolomics approach to compare the volatiles emitted by non-infected compost with those of infected compost. A proof of principle for this approach was obtained in an experimental model containing 300 g of freshly spawned, well aerated phase 2 compost. This model compared a development into fully infected compost with a normal spawn-run. In commercial practice of phase 3 composting, tunnels are likely partially infected. We therefore tested whether the method would also work in 50 kg batches of partially infected compost. The potential of using headspace volatiles asa detection method to detect T. aggressivumin the process air of the tunnels, avoiding the need to sample inside the tunnel is discussed.
机译：自2006年以来，绿色霉菌（Trichoderma aggressivum）给荷兰蘑菇业带来了大麻烦。侵略性山毛榉菌在很早的时候就感染堆肥，在荷兰，这种感染很可能发生在堆肥场。尽管荷兰的堆肥生产商非常热衷于预防绿色霉菌问题，但偶尔仍有许多农作物受到感染。因此，需要一种能够早期检测木霉绿色霉菌的方法。双孢蘑菇和侵略性T. agressiveum使用挥发物来影响彼此的生长速度。我们测试了使用产卵过程中产生的挥发物检测木霉绿色霉菌的可能性。我们使用了非目标代谢组学方法来比较未感染堆肥和被感染堆肥排放的挥发物。这种方法的原理证明是在一个实验模型中获得的，该模型包含300 g新鲜产生的，充气良好的2期堆肥。该模型将正常感染产生的堆肥发展为完全感染的堆肥。在第3阶段堆肥的商业实践中，隧道可能会受到部分感染。因此，我们测试了该方法是否也适用于50千克批次的部分感染堆肥。讨论了使用顶空挥发物作为检测方法来检测隧道过程空气中侵袭性三叶草的潜力，从而避免了在隧道内部进行采样的需要。

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