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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Genomics Changes Induced by Maitake D-Fraction in the Suppression of Breast Cancer Phenotype
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Genomics Changes Induced by Maitake D-Fraction in the Suppression of Breast Cancer Phenotype

机译：Maitake D分数诱导的乳腺癌表型的抑制引起的基因组学变化。

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It' s already known that Maitake (D-Fraction) mushroom is involved in stimulating the immune system and activating certain cells and proteins that attack cancer cells, including macrophages, T-cells, natural killer cells, and interleukin-1 and-2. For centuries, Japanese people have used Maitake mushrooms as a general promoter of robust health. According to the National Cancer Institute, polysaccharide complexes present in Maitake mushrooms appear to have significant anti-cancer activity. Also, it has been suggested that Maitake may work in conjunction with chemotherapy to lessen its side effects and to boost its positive effects. However, the exact molecular mechanism unchage by Maitake in the struggle against the cancerous cell is still unclear. At the present work we are trying to clarify the genomic expression induced by Maitake D-Fraction in MCF-7 breast cancer cells. We also want to demonstrate which genes are involved in the suppression of the breast tumoral phenotype and which biological pathways are involving in this effect. Therefore, we performed a new set of human cDNA microarrays assays including 25000 genes employing MCF-7 cells treated with and without increased doses of Maitake D-Fraction (91, 183 and 367 μg/mL), during 24 hours. The gene expressions were corroborated by real time RTPCR assay employing commercial available primers from Applied Biosystems, INC, CA. Understanding the molecular mechanisms induced by Maitake D-Fraction in the suppression of breast cancer phenotype may lead to the development of therapeutic/preventive strategies against highly incident of breast cancer.
机译：众所周知，舞茸（D-Fraction）参与刺激免疫系统并激活某些攻击癌细胞的细胞和蛋白质，包括巨噬细胞，T细胞，自然杀伤细胞以及白介素1和2。几个世纪以来，日本人一直使用舞茸作为促进健康的一般促进剂。根据国家癌症研究所的数据，舞茸中存在的多糖复合物似乎具有显着的抗癌活性。另外，有人建议舞茸可以与化学疗法一起使用，以减轻其副作用并增强其积极作用。但是，尚不清楚Maitake在对抗癌细胞方面的确切分子机制尚不清楚。在目前的工作中，我们试图阐明Maitake D-Fraction在MCF-7乳腺癌细胞中诱导的基因组表达。我们还想证明哪些基因参与了乳腺肿瘤表型的抑制，哪些生物学途径参与了这种效应。因此，我们在24小时内进行了一组新的人类cDNA微阵列测定，包括25000个基因，这些基因分别使用经过处理和未经处理的Maitake D-Fraction（91、183和367μg/ mL）处理的MCF-7细胞。通过使用来自Applied Biosystems，INC，CA的市售引物的实时RTPCR测定法证实了基因表达。了解Maitake D-Fraction在抑制乳腺癌表型中诱导的分子机制可能导致针对乳腺癌高发性的治疗/预防策略的发展。

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