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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Studies on Breeding of Shiitake Mushroom, Lentinula edodes, and Effect of Strain Preservation on Reproduction
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Studies on Breeding of Shiitake Mushroom, Lentinula edodes, and Effect of Strain Preservation on Reproduction

机译：香菇，香菇的繁殖及菌株保存对繁殖的影响研究

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The aim of this study was to investigate the longterm cultivar characteristics (fruiting body yield and sizes) in order to provide spawns for shiitake mushroom growers. The 6F1, 7F2, and 9F3 Shiitake mushroom cultivars were employed. Malt saccharified extract agar (MSA), MSA plus minerals (MSMA), potato dextrose agar (PDA) plus minerals plus corn steep liquor (PDMCA), and PDMCA plus Japanese oak sawdust (PDMCSA) were used as preservation media. Sawdust box culture was adopted (1.2 kg/box; Japanese oak sawdust plus wheat bran) for fruiting body cultivation. The fruiting body yield in the most mated dikaryotic mycelia with the same parental cultivar and stocked on MSA medium decreased by 50% in 1-2 years. During the spawn manufacturing process, repeated subculture on agar media also caused a decrease in yield. The use of different agar media and/or subculture frequency led to unevenness in yield. The fruiting body yield of the cultivar preserved on MSMA for 1 year was stable. Each cultivar required optimum preservation media for sustaining characteristics. Moreover, the parental cultivar and its F1 hybrids also required optimum preservation media. Isolating the fruiting body tissue is effective for sustaining cultivar characteristics. Thus, it is necessary to determine whether hybrids sustain their characteristics after 2 years of subculture, to choose the optimum preservation media for each cultivar, and to isolate cultivated fruiting body tissue.
机译：这项研究的目的是研究长期的栽培品种特性（子实体产量和大小），以便为香菇种植者提供产卵。使用了6F1、7F2和9F3香菇品种。麦芽糖化琼脂（MSA），MSA加矿物质（MSMA），马铃薯葡萄糖琼脂（PDA）加矿物质加玉米浆（PDMCA）和PDMCA加日本橡木锯末（PDMCSA）作为保存介质。采用木屑盒培养法（1.2公斤/盒；日本橡木木屑加麦麸）进行子实体培养。在具有相同亲本品种并在MSA培养基上饲养的最交配的原核生物菌丝体中，子实体产量在1-2年内下降了50％。在产卵过程中，在琼脂培养基上重复传代培养也会导致产量下降。使用不同的琼脂培养基和/或传代培养频率导致产量不均。在MSMA上保存1年的品种的子实体产量稳定。每个品种都需要最佳的保存介质来维持其特性。此外，亲本品种及其F1杂种还需要最佳的保存培养基。分离子实体组织对于维持品种特性是有效的。因此，有必要确定杂交体在继代培养2年后是否能维持其特性，为每个品种选择最佳的保存培养基，并分离出栽培的子实体组织。

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