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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Effect of Trace Element Copper Supplemented in Medium on Growth, Yield, and Physiology of Pleurotus eryngii
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Effect of Trace Element Copper Supplemented in Medium on Growth, Yield, and Physiology of Pleurotus eryngii

机译：补充微量元素铜对杏鲍菇生长，产量和生理的影响

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摘要

Trace element,copper,was added into the culture medium of Pleurotus eryngii at different concentrations for revealing effects of Cu on growth and physiology of P. eryngii. The growth rate of mycelium, quality of fruit body,and activity of some enzymes of P. eryngii were detected in various development periods. The fluxes of Ca2+, H+, and K+on the surface of mycelium cell were determined by NMT technology. The results show that the mycelium growth rate did not change under addition of Cu at the concentration of 20-100mg/kg,and the fruit body yield of P. eryngii was raised at a certain concentration range. When the concentration of Cu was 60mg/kg,the unit yield (169.18 g) of P. eryngii was the highest and it was 16.45% higher than that of the control group. At the concentration of 20-100mg/kg, Cu can promote the activities of nitrate reductase (NR), glutamate-pyruvate transaminase (GPT) and sucrose phosphate synthase (SPS) in P. eryngii. The extracellular H+flux was accelerated by adding Cu,the maximum flux reached to 0.587 pmol/(cm ? s) from budding period to harvesting period. The extracellular K+flux rate was accelerated at certain stage,but Cu at high concentrations inhibited the flux speed of K+. By adding Cu in medium, Ca2+changed from release state to absorption state at budding period, and was at absorption state during harvesting period.
机译：将微量元素铜以不同浓度添加到杏鲍菇的培养基中，以揭示铜对杏鲍菇生长和生理的影响。在不同的发育时期，检测了杏鲍菇菌丝体的生长速率，子实体质量和某些酶的活性。用NMT技术测定菌丝体细胞表面Ca2 +，H +和K +的通量。结果表明，添加浓度为20-100mg / kg的Cu，菌丝体的生长速率没有变化，在一定的浓度范围内，杏鲍菇的子实体产量提高。 Cu浓度为60mg / kg时，杏鲍菇的单产最高（169.18g），比对照组高16.45％。铜的浓度为20-100mg / kg时，可促进假单胞菌中硝酸还原酶（NR），谷氨酸-丙酮酸转氨酶（GPT）和蔗糖磷酸合酶（SPS）的活性。添加Cu可促进细胞外H +通量，从出芽期到收获期最大通量达到0.587 pmol /（cm·s）。细胞外K +通量在一定阶段被加速，但是高浓度的Cu抑制了K +的通量速度。通过在培养基中添加Cu，Ca2 +在出芽期从释放状态变为吸收状态，在收获期处于吸收状态。

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