首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >The Nuclear Ribosomal DNA Intergenic Spacer as a Target Sequence to Study Intraspecific Diversity of Auricularia auricula-judae
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The Nuclear Ribosomal DNA Intergenic Spacer as a Target Sequence to Study Intraspecific Diversity of Auricularia auricula-judae

机译:核糖体DNA基因间隔子作为研究Auricularia auricula-judae的种内多样性的目标序列。

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摘要

The complete nuclear rDNA gene complex of Auricularia auricula-judae isolate AU110 is 11210 bp long, contains the 18S, 5. 8S, and 28S rRNA gene as well as the ITS and IGS regions. Based on the sequence data, four species-specific primers combinations for amplified IGS region of A. auricula-judae were designed. Nucleotide sequence variation in the IGS among 32 A. auricula-judae commercial cultivars in China sorted into three strongly supported clades, which is correlated with geographical regions. Most strains originated from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains. The grouping information obtained in the present work provides significant information for further genetic improvement in A. auricula-judae, and suggested that IGS region can be used as an excellent tool for genetic polymorphism analysis.
机译:Auricularia auricula-judae分离株AU110的完整核rDNA基因复合体长11210 bp,包含18S,5.,8S和28S rRNA基因以及ITS和IGS区。根据序列数据,设计了四种木耳金葡菌IGS区域的特异性引物组合。在中国的32个极光金丝猴-商业品种中,IGS中的核苷酸序列变异分为三个强支持的进化枝,这与地理区域相关。来自同一地区的大多数菌株具有较窄的遗传基础,可能可以从当地的野生型菌株中驯化。在目前的工作中获得的分组信息提供了进一步的信息,以进一步改进金耳不动产,重要的信息,并建议IGS区域可以用作遗传多态性分析的优秀工具。

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