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首页> 外文期刊>Electronic Journal of Biotechnology >The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
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The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer

机译：利用核糖体DNA基因间隔子分析中国金耳木的分子多样性

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Background: For the crossbreeding of Auricularia auricula-judae, selecting the appropriated parents in hybridization is very important. However, the classification and diversity analysis of A. auricula-judae has been equivocal, due to the similarity of the fruiting body morphology and its susceptibility to environmental influences. For this purpose, the molecular diversity of 32 A. auricula-judae commercial cultivars in China was analyzed by using the nuclear ribosomal DNA intergenic spacer. Results: The complete nuclear rDNA gene complex of A. auricula-judae isolate is 11210 bp long, and contains the 18S, 5.8S, and 28S rRNA gene as well as the ITS and IGS regions. Based on the sequence data, four more effective primer combinations for the IGS region of A. auricula-judae were designed. Nucleotide sequence variation in the IGS among 32 A. auricula-judae commercial cultivars in China sorted into three strongly supported clades, which is correlated with geographical regions. Most strains originated from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains. Conclusion: The grouping information obtained in the present work provides significant information for further genetic improvement in A. auricula-judae, and suggested that the IGS region can be used as an excellent tool for identification of genetic variation.? 2014 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserved.
机译：背景：对于小木耳杂交，在杂交中选择合适的亲本非常重要。然而，由于子实体形态的相似性及其对环境影响的敏感性，金耳金针菇的分类和多样性分析一直是模棱两可的。为此，利用核糖核糖体DNA基因间隔子分析了中国32个极光金丝猴-商业品种的分子多样性。结果：金黄色葡萄球菌-judae分离株的完整核rDNA基因复合体长11210 bp，包含18S，5.8S和28S rRNA基因以及ITS和IGS区。基于序列数据，设计了四个更有效的引物组合，用于金合曲霉的IGS区域。在中国的32个极光金丝猴-商业品种中，IGS中的核苷酸序列变异分为三个强支撑进化枝，这与地理区域有关。来自同一地区的大多数菌株具有较窄的遗传基础，可能可以从当地的野生型菌株中驯化。结论：本研究获得的分组信息为进一步改进金耳不动产的遗传学提供了重要信息，并提示IGS区域可作为鉴定遗传变异的优良工具。 2014瓦尔帕莱索天主教大学。 Elsevier B.V.的制作和托管保留所有权利。

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