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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Optimization of Liquid Fermentation Conditions of Hirsutella sinensis by Response Surface Methodology
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Optimization of Liquid Fermentation Conditions of Hirsutella sinensis by Response Surface Methodology

机译：响应面法优化中华发酵菌的液体发酵条件。

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摘要

The quantitative effects of fermentation temperature, initial pH and inoculum volume on the yield of mycelial biomass and extracellular polysaccharide content produced by the fungus Hirsutella sinensisin in submerged fermentation were investigated using response surface methodology (RSM). The three factors chosen for the present investigation were based on the results of a previous Plackett-Burman (PB) design. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. RSM analysis showed good correspondence between experimental and predicted values. It was found that three parameters represented significant effect. The coefficient of determination (R2) for the model was 98. 8%. Probability value (P<0.0001) demonstrated a very high significance for the regression model. By solving the regression equation and also by analyzing the response surface contour plots, the optimal process parameters were determined: fermentation temperature 18. 31℃, initial pH 5.01 and inoculum volume 13.57mL. Under the optimal conditions, the maximum predicted yield of mycelium biomass was 18. 11 mg/mL, the yield of extracellular polysaccharide was 2. 03 mg/mL. Validation experiments verified the availability and the accuracy of the model.
机译：利用响应面法研究了发酵温度，初始pH值和接种量对沉水真菌Hirsutella sinensisin产生的菌丝生物量和胞外多糖含量的定量影响。本研究选择的三个因素是基于先前的Plackett-Burman（PB）设计的结果。使用多元回归分析将获得的实验数据拟合到二阶多项式方程，并通过适当的统计方法进行分析。 RSM分析显示实验值和预测值之间具有良好的对应关系。发现三个参数代表显着效果。该模型的测定系数（R2）为98.8％。概率值（P <0.0001）对于回归模型显示出非常高的意义。通过求解回归方程，并通过分析响应表面轮廓图，确定了最佳工艺参数：发酵温度18. 31℃，初始pH 5.01，接种量13.57mL。在最佳条件下，菌丝生物量的最大预测产量为18. 11 mg / mL，胞外多糖的产量为2. 03 mg / mL。验证实验验证了模型的可用性和准确性。

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