• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Statistical Optimization of Process Variables for the Production of Mycelial Biomass and Polysaccharides from Lentinus edodes
【24h】

Statistical Optimization of Process Variables for the Production of Mycelial Biomass and Polysaccharides from Lentinus edodes

机译：香菇菌丝体生物质和多糖生产过程变量的统计优化

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Physiologically-active polysaccharides are generally obtained from commercially cultivated mushroom fruiting bodies, which have a long cropping cycle. However, growing mushroom mycelia in a defined medium, by submerged fermentation is a rapid and alternative method to obtain fungal biomass of consistent quality for the extraction of polysaccharides (intracellular and extracellular). Response Surface Methodology (RSM) was used for statistically optimization of the process variables such as pH (4-8), temperature (15-351), incubation period (10-30 days) and agitation rate (46 -54 rpm) in liquid medium for maximum biomass production of 7 strains of L. edodes. Out of these, one strain LeI produced maximum biomass and polysaccharide. Central Composite Rotatable Design was applied to design experiments to evaluate the interactive effects of process variables using Design Expert, Trial version 8.0, Stat-Ease INC., Minneapolis, MN statistical software. The second order polynomial coefficients were calculated using the software and responses of the RSM were plotted on 3-D plots. The optimum operating conditions obtained by RSM were pH 5.0, temperature 21℃, incubation period 22 days and agitation rate 52. 0 rpm for maximum biomass (7.93 mg/mL) and polysaccharide (1. 67 mg/mL) production in LeI strain. These optimal conditions are being used for large scale fermentation for simultaneous production of biomass and polysaccharides.
机译：生理活性多糖通常得自商业种植的蘑菇子实体，其具有长的种植周期。然而，通过浸没式发酵在限定的培养基中生长蘑菇菌丝体是获得具有恒定质量的真菌生物质以提取多糖（细胞内和细胞外）的快速和替代方法。响应表面方法（RSM）用于统计优化过程变量，例如液体中的pH（4-8），温度（15-351），孵育时间（10-30天）和搅拌速率（46 -54 rpm）培养基可最大程度地生产7株香菇。在这些菌株中，一种菌株LeI产生最大的生物量和多糖。中央复合可旋转设计用于设计实验，以使用明尼苏达州明尼阿波利斯市Stat-Ease INC。设计专家8.0版试验专家评估过程变量的交互作用。使用软件计算二阶多项式系数，并将RSM的响应绘制在3-D图上。通过RSM获得的最佳操作条件是pH 5.0，温度21℃，孵育时间22天，搅拌速率52. 0 rpm，以使LeI菌株产生的最大生物量（7.93 mg / mL）和多糖（1. 67 mg / mL）。这些最佳条件被用于大规模发酵，以同时生产生物质和多糖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号