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首页> 外文期刊>Research Journal of Agriculture and Biological Sciences >Optimization of Submerged Culture Conditions for Mycelial Biomass Production by Shiitake Mushroom ( Lentinus edodes ).
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Optimization of Submerged Culture Conditions for Mycelial Biomass Production by Shiitake Mushroom ( Lentinus edodes ).

机译：香菇（Lentinus edodes）生产菌丝体生物质的深层培养条件的优化。

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摘要

Three strains of Lentinus edodes mushroom were grown using submerged culture. Different carbon andnitrogen sources were examined to determine the most suitable source and their optimum concentration forgrowth. Also, the effect of incubation time and temperature, pH value, and agitation were determined. Thehighest mycelial biomass yields of the tested strains were obtained when using a medium containing 70g /Lglucose, 4 g/ L yeast extract at 25 оC for 14 days, value pH 6.5 and agitation was 150 rpm. The mycelialbiomass obtained under the optimized conditions increased by 41.3, 40.6 and 31.2% compared to thoseharvested under the control conditions, for LC 0032, LC 236 and LC2161 strains, consecutively. LC 2161 wasmore efficient for producing the mycelial biomass compared to the other strains. Chemical constituents of themycelial biomass of the tested strains were estimated.
机译：使用浸没培养法培养了三株香菇。检查了不同的碳和氮源，以确定最合适的源及其最佳生长浓度。此外，确定了孵育时间和温度，pH值和搅拌的影响。当使用含有70g / L葡萄糖，4 g / L酵母提取物的培养基在25℃下发酵14天，pH值为6.5，搅拌速度为150 rpm时，菌株的菌丝体生物量最高。对于LC 0032，LC 236和LC2161菌株，在最佳条件下获得的菌丝体生物量比在对照条件下收获的菌丝体生物量连续增加了41.3％，40.6％和31.2％。与其他菌株相比，LC 2161更有效地生产菌丝体生物质。估计了测试菌株的菌丝体生物量的化学成分。

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