Cloning and Characterization of NADPH-cytochrome P450 Reductase in the Cotton Bollworm,Helicoverpa armigera

机译：棉铃虫NADPH-细胞色素P450还原酶的克隆与鉴定

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@@ Previous studies have showed that cytochrome P450 CYP6B7 plays an important role in the pyrethroidresistance of Helicoverpa armigera. As known to all,CPR plays an essential role in the transfer of reducing electron from NADPH to cytochrome P450 enzyme systems (P450s). Some studies have suggested that CPR may be involved in the resistance of insect to insecticide. However, little information about CPR in H. armigera has been reported. In this study, the CPR gene of H. armigera was cloned and characterized through analysis on bioinformatics, spatial and developmental expression and phenobarbital (PB) induction.

机译：以前的研究表明，细胞色素P450 CYP6B7在棉铃虫的拟除虫菊酯抗性中起重要作用。众所周知，CPR在还原电子从NADPH转移到细胞色素P450酶系统（P450s）中起着至关重要的作用。一些研究表明，CPR可能与昆虫对杀虫剂的抗性有关。但是，关于棉铃虫心肺复苏术的报道很少。在这项研究中，通过分析生物信息学，空间和发育表达以及苯巴比妥（PB）诱导，克隆了棉铃虫CPR基因并对其进行了表征。

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来源
《Proceedings of 4th international symposium on pesticides and environmental safety amp; 5th pan Pacific confernce on pesticide science amp; 8th international workshop on crop protection chemistry and regulatory harmonization》|2012年|418-419|共2页
会议地点 Beijing(CN)
作者
Chunqing Zhao; Tao Tang; Xiaoyun Feng; Lihong Qiu;
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作者单位

College of Science, China Agricultural University, Beijing, China;

College of Science, China Agricultural University, Beijing, China;

College of Science, China Agricultural University, Beijing, China;

College of Science, China Agricultural University, Beijing, China;

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会议组织
原文格式 PDF
正文语种 eng
中图分类植物化学保护理论;农用化学物质、有毒化学物质污染及其防治;
关键词
NADPH-cytochrome P450 reductase; Helicoverpa armigera; real-time quantitative polymerase chain reaction; phenobarbital; cytochrome P450 enzyme systems;

机译：NADPH-细胞色素P450还原酶；棉铃虫；实时定量聚合酶链反应；苯巴比妥；细胞色素P450酶系统;

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专利

1. Characterization of NADPH-cytochrome P450 reductase gene from the cotton bollworm, Helicoverpa armigera [J] . LiuD., ZhouX., LiM., Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2014,第2期

机译：棉铃虫NADPH-细胞色素P450还原酶基因的表征
2. Cloning and characterisation of NADPH-dependent cytochrome P450 reductase gene in the cotton bollworm, Helicoverpa armigera [J] . Chunqing Zhao, Tao Tang, Xiaoyun Feng Pest Management Science . 2014,第1期

机译：棉铃虫NADPH依赖性细胞色素P450还原酶基因的克隆与鉴定
3. Effect of a functional recombinant cytochrome P450 enzyme of Helicoverpa armigera on gossypol metabolism co-expressed with NADPH-cytochrome P450 reductase in Pichia pastoris [J] . Chen Cheng, Pi Wenhui, Zhang Yan, Pesticide Biochemistry and Physiology . 2019,第Mara期

机译：Helicoverpa armigera官能团重组细胞色素P450酶对毕赤酵母中NADPH-细胞色素P450还原酶CON-COMICOLARIS的影响
4. Cloning and Characterization of NADPH-cytochrome P450 Reductase in the Cotton Bollworm,Helicoverpa armigera [C] . Chunqing Zhao, Tao Tang, Xiaoyun Feng, International workshop on crop protection chemistry and regulatory harmonization . 2012

机译：棉铃虫中NADPH-细胞色素P450还原酶的克隆与表征，Helicoverpa Armigera
5. The influence of environmental factors on susceptibility of B.t. cottons to bollworm, Helicoverpa zea, and factors affecting resistance to B.t. toxins for bollworm. [D] . Burd, Anthony David. 2001

机译：环境因素对B.t.棉铃虫，Helicoverpa zea以及影响抗B.t.棉铃虫的毒素。
6. Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera [O] . Cheng Chen, Yan Zhang, Wenhui Pi, 2019

机译：利用棉铃虫功能性重组NADPH-细胞色素P450还原酶和细胞色素P450CYP9A12降低棉粕中棉酚含量的工艺参数优化
7. Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera [O] . Cheng Chen, Yan Zhang, Wenhui Pi, 2019

机译：通过功能重组NADPH-细胞色素P450还原酶P450还原酶对棉籽粕中棉毒水平降低的过程参数优化，Helicoverpa armigera的细胞色素P450 CYP9A12

Cloning and Characterization of NADPH-cytochrome P450 Reductase in the Cotton Bollworm,Helicoverpa armigera

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