TISSUE-ENGINEERED CARTILAGE IN THREE-DIMENSIONAL CULTURED CHONDROCYTES BY ULTRASOUND STIMULATION AND COLLAGEN SPONGE AS SCAFFOLD

机译：超声刺激和胶原海绵作为支架在三维培养的软骨细胞中组织工程化软骨

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This paper describes the effects of ultrasound stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen sponges as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis). It has been shown that cell proliferation and matrix production can be facilitated by considering the mechanical environment of the cultured chondrocytes and the mechanical properties of the scaffold structure used in the culture and of the stimulation used.

机译：本文描述了超声刺激对三维培养中软骨细胞与再生软骨组织产生的关系，该过程使用胶原海绵作为载体并补充透明质酸（用于骨关节炎的保守治疗）。已经表明，通过考虑培养的软骨细胞的机械环境以及用于培养的支架结构的机械性能和所使用的刺激，可以促进细胞增殖和基质产生。

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来源
《Proceedings of the ASME international mechanical engineering congress and exposition 2009》|2009年|P.463-464|共2页
会议地点 Lake Buena Vista FL(US);Lake Buena Vista FL(US)
作者
Toshihiko Shiraishi; letomo Matsunaga; Shin Morishita; Ryohei Takeuchi; Tomoyuki Saito; Yuko Mikuni-Takagaki;
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Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan;

rnGraduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan;

rnGraduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan;

rnDepartment of Orthopedic Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;

rnDepartment of Orthopedic Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;

rnDepartment of Biochemistry and Molecular Biology, Kanagawa Dental College, 82 Ineoka-cho, Yokosuka 238-8580;

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正文语种 eng
中图分类机械设计、计算与制图;
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1. BMP-2 embedded atelocollagen scaffold for tissue-engineered cartilage cultured in the medium containing insulin and triiodothyronine-a new protocol for three-dimensional in vitro culture of human chondrocytes [J] . KoE.C., FujiharaY., OgasawaraT., Tissue engineering, Part C. Methods . 2012,第5期

机译：BMP-2包埋的端粒胶原蛋白支架，用于在含有胰岛素和三碘甲腺嘌呤的培养基中培养的组织工程软骨-一种新的人软骨细胞三维体外培养方案
2. Enhanced hyaline cartilage matrix synthesis in collagen sponge scaffolds by using siRNA to stabilize chondrocytes phenotype cultured with bone morphogenetic protein-2 under hypoxia [J] . LegendreF., OllitraultD., HervieuM., Tissue engineering, Part C. Methods . 2013,第7期

机译：缺氧条件下使用siRNA稳定骨形态发生蛋白2培养的软骨细胞表型，从而增强胶原海绵支架中的透明软骨基质合成
3. Cartilage formation by serial passaged cultured chondrocytes in a new scaffold: hybrid 75:25 poly(L-lactide-epsilon-caprolactone) sponge. [J] . Honda MJ, Yada T, Ueda M, Journal of Oral and Maxillofacial Surgery . 2004,第12期

机译：在新的支架中，通过连续传代培养的软骨细胞形成软骨：杂化的75:25聚（L-丙交酯-ε-己内酯）海绵。
4. TISSUE-ENGINEERED CARTILAGE IN THREE-DIMENSIONAL CULTURED CHONDROCYTES BY ULTRASOUND STIMULATION AND COLLAGEN SPONGE AS SCAFFOLD [C] . Toshihiko Shiraishi?, Ietomo Matsunaga, Shin Morishita International Mechanical Engineering Congress and Exposition . 2010

机译：通过超声刺激和胶原海绵的三维培养的软骨细胞中的组织工程软骨
5. Role of scaffold topography and stimulation via ultrasound on the biosynthetic activity of chondrocytes seeded in three-dimensional matrices. [D] . Noriega, Sandra Edith. 2009

机译：支架形貌和超声刺激对播种在三维矩阵中的软骨细胞的生物合成活性的作用。
6. Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold [O] . Koji Hattori, Yoshinori Takakura, Hajime Ohgushi, 2005

机译：定量超声可以使用粘附的骨髓细胞和三维支架之间的复合物评估组织工程软骨的再生过程
7. Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold [O] . Hattori, Koji, Takakura, Yoshinori, Ohgushi, Hajime, 2005

机译：定量超声可以使用粘附的骨髓细胞和三维支架之间的复合物来评估组织工程软骨的再生过程

TISSUE-ENGINEERED CARTILAGE IN THREE-DIMENSIONAL CULTURED CHONDROCYTES BY ULTRASOUND STIMULATION AND COLLAGEN SPONGE AS SCAFFOLD

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