A Simple Automated Microfluidic System for Rapid Capillary Electrophoretic Separation of DNA Fragments

机译：一个简单的自动化微流控系统，用于快速毛细管电泳分离DNA片段

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摘要

An automated microfluidic system with laser induced fluorescence (LIF) detection for rapid capillary electrophoretic separation of DNA fragments is presented. The system was developed based on the hyphenation of a high throughput nanoliter sample introduction system and liquid-core waveguide (LCW) technique to produce the simple microfluidic system of high throughput, low consumption for achieving capillary electrophoretic separation of DNA fragments.

机译：提出了一种具有激光诱导荧光（LIF）检测的自动微流控系统，用于快速毛细管电泳分离DNA片段。该系统是基于高通量纳升样品引入系统的联用和液芯波导（LCW）技术而开发的，以产生高通量，低消耗量的简单微流体系统，以实现DNA片段的毛细管电泳分离。

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来源
《Proceedings of the First Shenyang International Colloquium on Microfluidies》|2007年|155-156|共2页
会议地点 Shenyang(CN);Shenyang(CN)
作者
F.Benjamin Zhan; rnXuwei Chen;
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作者单位

Xiao-Feng Fan@Research Center for Analytical Sciences, Northeastern University, Shenyang, China--Zhang-Run Xu@Research Center for Analytical Sciences, Northeastern University, Shenyang, China--Qi Li, Shi-Li Wang@Research Center for Analytical Sciences, Northeastern University, Shenyang, China--Zhao-Lun Fang@Research Center for Analytical Sciences, Northeastern University, Shenyang, China--;

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会议组织
原文格式 PDF
正文语种 eng
中图分类射流技术（流控技术）;
关键词
Microfluidic; Capillary electrophoresis; DNA rnseparation;

机译：微流控毛细管电泳DNA分离;

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专利

1. A simple microfluidic system for efficient capillary electrophoretic separation and sensitive fluorimetric detection of DNA fragments using light-emitting diode and liquid-core waveguide techniques [J] . Wang SL, Fan XF, Xu ZR, Electrophoresis: The Official Journal of the International Electrophoresis Society . 2005,第19期

机译：使用发光二极管和液芯波导技术进行高效毛细管电泳分离和灵敏的荧光检测DNA片段的简单微流控系统
2. Rapid and reproducible capillary electrophoretic separation of double-stranded DNA fragments in a simple methyl cellulosic sieving system [J] . Roed L., Lundanes E., Greibrokk T., Chromatographia . 1998,第3a4期

机译：在简单的甲基纤维素筛分系统中快速且可重现的毛细管电泳分离双链DNA片段
3. Sequence-specific nucleic acid mobility using a reversible block copolymer gel matrix and DNA amphiphiles (lipid-DNA) in capillary and microfluidic electrophoretic separations [J] . Wagler Patrick, Minero Gabriel Antonio S., Tangen Uwe, Electrophoresis: The Official Journal of the International Electrophoresis Society . 2015,第19期

机译：使用可逆嵌段共聚物凝胶基质和DNA两亲物（脂质DNA）在毛细管和微流控电泳分离中进行序列特异性核酸迁移
4. A Simple Automated Microfluidic System for Rapid Capillary Electrophoretic Separation of DNA Fragments [C] . Shenyang International Colloquium on Microfluidies . 2007

机译：一种简单的自动微流体系统，用于DNA片段的快速毛细管电泳分离
5. Solid-phase DNA sequencing reactions performed in micro-capillary reactors and solid-phase reversible immobilization in microfluidic chips for purification of dye-labeled DNA sequencing fragments. [D] . Xu, Yichnan. 2003

机译：在微毛细管反应器中进行固相DNA测序反应，并在微流控芯片中进行固相可逆固定，以纯化染料标记的DNA测序片段。
6. Integrated Microfluidic Device for Automated Single Cell Analysis using Electrophoretic Separation and Electrospray Ionization Mass Spectrometry [O] . J. Scott Mellors, Kaveh Jorabchi, Lloyd M. Smith, -1

机译：采用电泳分离和电喷雾质谱法集成微流体装置自动单细胞分析
7. Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions [O] . Braun, B., Blanch, W., Prausnitz, J.M. 1997

机译：使用稀释的聚合物溶液毛细管电泳分离DNa限制性片段
8. Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions [R] . Braun, B. , Blanch, W. , Prausnitz, J. M. 1997

机译：使用稀释的聚合物溶液毛细管电泳分离DNa限制性片段

A Simple Automated Microfluidic System for Rapid Capillary Electrophoretic Separation of DNA Fragments

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