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Chromosome Variation from Sect.Chinenses (Rosa L.) through Chinese Old Garden Roses to Modern Rose Cultivars

机译:从中国科植物玫瑰到中国现代玫瑰品种的染色体变异

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摘要

Contributing recurrent flowering and tea scent to modern roses, wild Rosa resources from China especially Rosa chinensis and R.odorata complex, and Chinese Old Garden Roses have played very important roles in the formation history of modern roses.In order to understand the chromosome variation in the formation process of modern roses, karyological studies of 6 species/varieties from Sect.Chinenses, 9 Chinese Old Garden Roses and 5 modern roses were carried out by traditional squashing and pressing.Their molecular cytogenetic characters were analyzed with FISH using 45S rDNA as probes.The results were the following:1) species/varieties from Sect.Chinenses were all diploid with 14 chromosomes,except the triploid R.ododrata var.erubescens from Lijiang.Except for R.chinensis 'Tiebahong' with a karyotype of 2A, all the others belonged to 1A.Two 45S rDNA hybridization sites were observed in the pro-metaphase and interphase cells of diploid plants, while three sites were found in those of triploid R.odorata var.erubescens; 2) there were 3 pioidies, namely diploidy, triploidy and tetraploidy, and 4 karyotypes, namely 1A, 2A, 1B and 2B, in the Old Garden Roses.Their 45S rDNA hybridization sites were also coincident with their ploidy, but the intensity of hybridization signals differed much among cultivars; 3) all the studied modern roses were tetraploid, but they had only three 45S rDNA hybridization sites.Their FISH signals were unanimous in size and intensity.The results showed that Chinese Old Garden Roses got more variable in chromosome number and structure and became more diverse in number, size and intensity of 45S rDNA FISH signals than their original progenitors from Sect.Chinenses.However, modern roses got more homogenous in cytogenetics because of the repeated hybridization and inbreeding among cultivars.It is necessary to introgress wild species and Old Garden Roses into modern roses for improvement.
机译:经常性开花和茶香为现代玫瑰的发展提供了条件,来自中国的野生蔷薇资源,特别是中国的蔷薇和蔷薇科植物,以及中国的老花园玫瑰在现代玫瑰的形成历史中起着非常重要的作用。现代玫瑰的形成过程,中国科学院的6种/变种,传统的压榨和压榨进行了6种/变种的染色体核学研究,用FISH分析了它们的分子细胞遗传学特征,使用45S rDNA作为探针结果如下:1)来自丽江科的中华种/变种均为14染色体的二倍体,除了来自丽江的三倍体R.ododrata var.erubescens外,除了核型为2A的中华铁T(T.bahong)二倍体植物的前中期和间期细胞中观察到两个45S rDNA杂交位点,而三倍体植物中发现了三个位点。倍体红景天2)老花园玫瑰中有3个二倍体,三倍体和四倍体pioidies,以及4A核型,即1A,2A,1B和2B。它们的45S rDNA杂交位点也与它们的倍性一致,但杂交强度品种之间的信号差异很大; 3)研究的所有现代玫瑰均为四倍体,但只有3个45S rDNA杂交位点,其FISH信号在大小和强度上均一致,结果表明,中国老园林玫瑰的染色体数目和结构具有更多的变异性,并且具有更多的多样性45S rDNA FISH信号在数量,大小和强度上都比中国扇贝原始祖细胞高,但是由于品种之间的反复杂交和近交,现代玫瑰在细胞遗传学上变得更加同质,有必要使野生种和老花园玫瑰渗入改进现代玫瑰。

著录项

  • 来源
  • 会议地点 Beijing(CN)
  • 作者单位

    Yunnan Key Laboratory for Flower Breeding/Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China;

    Yunnan Key Laboratory for Flower Breeding/Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China;

    Yunnan Agricultural University, Kunming 650201, China;

    Yunnan Key Laboratory for Flower Breeding/Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China;

    Yunnan Key Laboratory for Flower Breeding/Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China;

    Yunnan Key Laboratory for Flower Breeding/Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China;

    Yunnan Key Laboratory for Flower Breeding/Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 观赏园艺(花卉和观赏树木);
  • 关键词

    Rosa chinensis; R.odorata; karyotype; fluorescence in situ hybridization (FISH); 45S rDNA;

    机译:蔷薇;臭odor;核型;荧光原位杂交; 45S rDNA;

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