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首页> 外文会议>Proceedings of the international conference on germplasm of ornamentals >Establishment of a Regeneration System for the Genetic Transformation of Ground-Cover Chrysanthemum 'Jindinghongxin'
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Establishment of a Regeneration System for the Genetic Transformation of Ground-Cover Chrysanthemum 'Jindinghongxin'

机译：地上菊花“金顶红心”遗传转化再生体系的建立

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Ground-cover Chrysanthemum has been used in gardens due to its rich colors,excellent ornamental value.Too much precipitation in summer of north China limits its further use.In order to create chrysanthemum with high resistance to waterlogging, the Vgb (Vitreoscilla hemoglobin) and pmi (phosphomannose isomerase)genes are transferred into the genome of ground-cover chrysanthemum 'Jindinghongxin' by Agrobacterium tumefaciens mediated transformation in our research.This research is of profound importance to broaden the usage of ground-cover chrysanthemum in gardens.This research optimized the regeneration system of 'Jindinghongxin'including choosing young leaves as explants and the best regeneration culture medium (MS+2.0 mg/L 6-BA+0.5 mg/L NAA), Transformation system for 'Jindinghongxin' was established by pre-culturing leaf explants for 2 days.The suitable concentration of Agrobacterium tumefaciens and the suitable dipping time for transformation was OD600=0.4 for 5 minutes.Co-cultivation was done for 2 days at 28℃ in the dark after infection and the explants were then put in delayed culture for 4 days.Then the explants were cultured in first selection medium with 6 g/L mannose for 15 days, and then cultured in second selection medium with 7 g/L mannose.Once the regeneration shoot was 1 cm long it was transformed into MS with 350 mg/L carbenicillin for root induction.
机译：地被植物菊花由于其色彩丰富，极好的观赏价值而被用于花园。华北地区夏季降水过多限制了它的进一步应用。为了产生对水渍具有高抵抗力的菊花，Vgb（玻璃体血红蛋白）和根癌农杆菌介导的转化将pmi（磷酸甘露糖异构酶）基因转移到地表菊花'金顶红新'的基因组中，这项研究对于拓宽地表菊花在花园中的应用具有重要意义。选择幼嫩叶片作为外植体和最佳再生培养基（MS + 2.0 mg / L 6-BA + 0.5 mg / L NAA）的“金鼎红新”再生体系，通过预培养叶片外植体建立“金鼎红新”转化体系共培养2天，合适的根癌土壤杆菌浓度和合适的浸入时间为OD600 = 0.4 5分钟。在感染后于黑暗中于28℃下进行2天，然后将外植体延迟培养4天。然后将外植体在含有6 g / L甘露糖的第一选择培养基中培养15天，然后在第二次选择培养基含7 g / L甘露糖。再生芽长1 cm时，将其转化为含350 mg / L羧苄青霉素的MS以诱导根。

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