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首页> 外文期刊>Acta Horticulturae >Establishment of a regeneration system for the genetic transformation of ground-cover Chrysanthemum 'Jindinghongxin'.
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Establishment of a regeneration system for the genetic transformation of ground-cover Chrysanthemum 'Jindinghongxin'.

机译：建立地被菊花“金顶红心”的遗传转化再生体系。

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Ground-cover Chrysanthemum has been used in gardens due to its rich colors, excellent ornamental value. Too much precipitation in summer of north China limits its further use. In order to create chrysanthemum with high resistance to waterlogging, the Vgb (Vitreoscilla hemoglobin) and pmi (phosphomannose isomerase) genes are transferred into the genome of ground-cover chrysanthemum 'Jindinghongxin' by Agrobacterium tumefaciens mediated transformation in our research. This research is of profound importance to broaden the usage of ground-cover chrysanthemum in gardens. This research optimized the regeneration system of 'Jindinghongxin' including choosing young leaves as explants and the best regeneration culture medium (MS+2.0 mg/L 6-BA+0.5 mg/L NAA), Transformation system for 'Jindinghongxin' was established by pre-culturing leaf explants for 2 days. The suitable concentration of Agrobacterium tumefaciens and the suitable dipping time for transformation was OD600=0.4 for 5 minutes. Co-cultivation was done for 2 days at 28 degrees C in the dark after infection and the explants were then put in delayed culture for 4 days. Then the explants were cultured in first selection medium with 6 g/L mannose for 15 days, and then cultured in second selection medium with 7 g/L mannose. Once the regeneration shoot was 1 cm long it was transformed into MS with 350 mg/L carbenicillin for root induction.
机译：地上菊由于其色彩丰富，极具观赏价值而被用于花园。华北地区夏季降水过多，限制了其进一步利用。为了创建对水涝具有高抗性的菊花，在我们的研究中，将Vgb（玻璃体玻璃血红蛋白）和pmi（磷酸甘露糖异构酶）基因转移到地面覆盖的菊花``金顶红新''基因组中。这项研究对于扩大菊科菊花在花园中的应用具有重要意义。本研究通过选择幼叶为外植体和最佳再生培养基（MS + 2.0 mg / L 6-BA + 0.5 mg / L NAA）优化了“金鼎红新”的再生体系，通过预先建立了“金鼎红新”的转化体系。 -培养叶外植体2天。适宜的根癌土壤杆菌浓度和浸入时间为OD 600 = 0.4，持续5分钟。感染后在黑暗中于28摄氏度共培养2天，然后将外植体延迟培养4天。然后将外植体在具有6 g / L甘露糖的第一选择培养基中培养15天，然后在具有7 g / L甘露糖的第二选择培养基中培养。再生芽长1厘米后，将其转化为含350 mg / L羧苄青霉素的MS，用于诱导根。

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