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首页> 外文会议>Proceedings of Life in space for life on earth >PROTEOMIC ANALYSIS OF HAIR FOLLICLES
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PROTEOMIC ANALYSIS OF HAIR FOLLICLES

机译：毛囊的蛋白质组学分析

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Hair root cells actively divide in a hair follicle, and theyrnsensitively reflect physical conditions. By analyzing thernhuman hair, we can know stress levels on the humanrnbody and metabolic conditions caused by microgravityrnenvironment and cosmic radiation. The Japan AerospacernExploration Agency (JAXA) has initiated a humanrnresearch study to investigate the effects of long-termrnspace flight on gene expression and mineral metabolismrnby analyzing hair samples of astronauts who stayed inrnthe International Space Station (ISS) for 6 months.rnDuring long-term flights, the physiological effects onrnastronauts include muscle atrophy and bone calcium loss.rnFurthermore, radiation and psychological effects arernimportant issue to consider. Therefore, an understandingrnof the effects of the space environment is important forrndeveloping countermeasures against the effectsrnexperienced by astronauts.rnIn this experiment, we identify functionally importantrntarget proteins that integrate transcriptome, mineralrnmetabolism and proteome profiles from human hair. Torncompare the protein expression data with the genernexpression data from hair roots, we developed the proteinrnprocessing method. We extracted the protein from fivernstrands of hair using ISOGEN reagents. Then, thesernextracted proteins were analyzed by LC-MS/MS. Theserncollected profiles will give us useful physiologicalrninformation to examine the effect of space flight.
机译：发根细胞活跃地分裂在毛囊中，并敏感地反映身体状况。通过分析人的头发，我们可以知道人体的压力水平以及微重力环境和宇宙辐射所引起的代谢状况。日本宇宙航空研究开发机构（JAXA）已启动一项人体研究，以通过分析在国际空间站（ISS）停留6个月的宇航员的头发样本来研究长期太空飞行对基因表达和矿物质代谢的影响。 ，对神经营养不良的生理学影响包括肌肉萎缩和骨钙流失。此外，辐射和心理影响也是要考虑的重要问题。因此，了解太空环境的影响对于开发针对宇航员所经历的影响的对策非常重要。在本实验中，我们确定了功能上重要的靶蛋白，这些蛋白整合了人类头发的转录组，矿物质代谢和蛋白质组图谱。通过比较发根中的蛋白质表达数据和基因表达数据，我们开发了蛋白质处理方法。我们使用ISOGEN试剂从头发的五链中提取了蛋白质。然后，通过LC-MS / MS分析提取的蛋白质。这些收集的资料将为我们提供有用的生理信息，以检查太空飞行的影响。

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    Noriaki Ishioka; Masahiro Terada; Shin Yamada; Masaya Seki; Rika Takahashi; Hideyuki J. Majima; Akira Higashibata; Chiaki Mukai;

  • 作者单位

    Japan Aerospace Exploration Agency, 2-1-1, Sengen, Tsukuba, Ibaraki, 305-8505, Japan, shioka.noriaki@jaxa.jp;

    Japan Aerospace Exploration Agency, 2-1-1, Sengen, Tsukuba, Ibaraki, 305-8505, Japan,terada.masahiro@jaxa.jp;

    Japan Aerospace Exploration Agency, 2-1-1, Sengen, Tsukuba, Ibaraki, 305-8505, Japan, amada.shin@jaxa.jp,;

    Advanced Engineering Services Co., Ltd, 1-6-1, Takezono, Tsukuba, Ibaraki, 305-0032, Japan,seki.masaya@jaxa.jp;

    Advanced Engineering Services Co., Ltd, 1-6-1, Takezono, Tsukuba, Ibaraki, 305-0032, apan,r_takahashi@aes.co.jp;

    Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan, hmajima@dent.kagoshima-u.ac.jp;

    Japan Aerospace Exploration Agency, 2-1-1, Sengen, Tsukuba, Ibaraki, 305-8505, Japan,higashibata.akira@jaxa.jp;

    Japan Aerospace Exploration Agency, 2-1-1, Sengen, Tsukuba, Ibaraki, 305-8505, Japan,mukai.chiaki@jaxa.jp;

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