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首页> 外文会议>Proceedings of Life in space for life on earth >RESPONSES OF MYOSIN HEAVY CHAIN PHENOTYPES AND GENE EXPRESSIONS IN NECK MUSCLE TO MICRO- AND HYPER-GRAVITY IN MICE
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RESPONSES OF MYOSIN HEAVY CHAIN PHENOTYPES AND GENE EXPRESSIONS IN NECK MUSCLE TO MICRO- AND HYPER-GRAVITY IN MICE

机译：小鼠肌球蛋白重链表型和基因表达对小鼠超微重力的反应

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摘要

Neck muscles are known to play important roles in thernmaintenance of head posture against gravity. However,rnit is not known how the properties of neck muscle arerninfluenced by gravity. Therefore, the current study wasrnperformed to investigate the responses of neck musclern(rhomboideus capitis) in mice to inhibition of gravityrnand/or increase to 2-G for 3 months to test the hypothesisrnthat the properties of neck muscles are regulated inrnresponse to the level of mechanical load applied by therngravitational load. Three male wild type C57BL/10Jrnmice (8 weeks old) were launched by space shuttlernDiscovery (STS-128) and housed in JapanesernExperimental Module “KIBO” on the InternationalrnSpace Station in mouse drawer system (MDS) project,rnwhich was organized by Italian Space Agency. Only 1rnmouse returned to the Earth alive after 3 months byrnspace shuttle Atlantis (STS-129). Neck muscles werernsampled from both sides within 3 hours after landing.rnCage and laboratory control experiments were also performed on the ground. Further, 3-monthrnground-based control experiments were performed withrn6 groups, i.e. pre-experiment, 3-month hindlimbrnsuspension, 2-G exposure by using animal centrifuge,rnand vivarium control (n=5 each group). Five mice werernallowed to recover from hindlimb suspension (includingrn5 cage control) for 3 months in the cage. Neck musclesrnwere sampled bilaterally before and after 3-monthrnsuspension and 2-G exposure, and at the end of 3-monthrnambulation recovery. Spaceflight-associated shift ofrnmyosin heavy chain phenotype from type I to II andrnatrophy of type I fibers were observed. In response tornspaceflight, 17 genes were up-regulated and 13 genesrnwere down-regulated vs. those in the laboratory control.rnExpression of 6 genes were up-regulated and that of 88rngenes were down-regulated by 3-month exposure to 2-Grnvs. the age-matched cage control. In response tornchronic hindlimb suspension, 4 and 20 genes were up- orrndown-regulated. Further, 98 genes respondedrnsignificantly to both hindlimb unloading and exposure torn2-G. Thirteen genes were up-regulated and 85 wererndown-regulated. In conclusion, long-term gravitationalrnunloading of mouse caused shift of fiber phenotyperntoward fast-twitch type and atrophy of slow-twitch fibersrnin neck muscle. These responses were closely relatedrnto the up- or down-regulation of genes, suggesting thatrnoxidative muscular metabolism may be inhibited inrnmicrogravity environment.
机译：已知颈部肌肉在保持头部姿势对抗重力方面起着重要作用。然而，未知如何通过重力来影响颈部肌肉的特性。因此，本研究旨在研究小鼠颈部肌肉对菱形的抑制作用和/或增加至2-G持续3个月的反应，以检验以下假设：调节颈部肌肉的特性对机械水平的反应不大。由重力载荷施加的载荷。由航天飞机发现号（STS-128）发射了三只雄性野生型C57BL / 10Jrnmice（8周龄），并安置在国际空间站老鼠抽屉系统（MDS）项目中的日语实验模块“ KIBO”中，该项目由意大利航天局组织。经过3个月的亚特兰蒂斯号航天飞机（STS-129），只有1鼠活着回到地球。着陆后3小时内从两侧取样颈部肌肉。还在地面上进行了笼子和实验室控制实验。此外，对6个组进行了3个月的基于地面的对照实验，即实验前，3个月后肢悬浮，通过使用动物离心机进行的2-G暴露，rn和饲养箱对照（每组n ＝ 5）。吞咽五只小鼠，使其在笼中从后肢悬浮液中恢复（包括笼控制），持续3个月。在3个月的悬吊和2-G暴露之前和之后以及3个月的恢复运动结束时，双侧取样颈部肌肉。观察到与空间飞行相关的rnmyosin重链表型从I型转变为II型和I型纤维的萎缩。与实验室对照相比，太空飞行中有17个基因被上调，而13个基因被下调。暴露于2-Grnvs中3个月，有6个基因的表达被上调，而88个基因的表达则被下调。年龄匹配的笼式控制。为了响应后肢的非正常悬吊，向上和向下调节了4和20个基因。此外，有98个基因对后肢的卸载和暴露于rn2-G的反应显着。上调了13个基因，下调了85个基因。综上所述，小鼠长期重力失重导致纤维表型向快速抽动型转移，并导致颈部颈部缓慢抽动性纤维萎缩。这些反应与基因的上调或下调密切相关，提示氧化性肌肉代谢可能在微重力环境中受到抑制。

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  • 会议地点 Aberdeen(GB)
  • 作者

    Tomotaka Ohira; Takashi Ohira; F. Kawano; T. Shibaguchi; H. Okabe; Y. Ohno; N. Nakai; T. Ochiai; K. Goto; Y. Ohira;

  • 作者单位

    Department of Physiology, School of Health Sciences, Toyohashi SOZO University, 20-1 Matsushita, Ushikawa, Toyohashi, Aichi 440-8511, Japan, Email: s1255101@sc.sozo.ac.jp;

    Graduate School of Frontier Biosciences, Osaka University, Toyonaka City, Osaka 560-0043, Japan,Email: ohhira.takashi@jaxa.jp;

    Graduate School of Medicine, Osaka University, Toyonaka City, Osaka 560-0043, Japan,Email: kawaco@space.hss.osaka-u.ac.jp;

    Graduate School of Frontier Biosciences, Osaka University, Toyonaka City, Osaka 560-0043, Japan,Email: shibaguchi@space.hss.osaka-u.ac.jp;

    Faculty of Letters, Kokushikan University, Setagaya-ku, Tokyo 154-0017, Japan,E-mail: rie-okah-sc220@m2.gyao.ne.jp;

    Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, 20-1 Matsushita, Ushikawa, Toyohashi, Aichi 440-8511, Japan, Email: yohno@sozo.ac.jp;

    Graduate School of Medicine, Osaka University, Toyonaka City, Osaka 560-0043, Japan,Email: nakai@hss.osaka-u.ac.jp;

    Mitsubishi Heavy Industry, Kobe, Hyogo 652-8585, Japan, E-mail: toshimasa_ochiai@mhi.co.jp;

    Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, 20-1 Matsushita, Ushikawa, Toyohashi, Aichi 440-8511, Japan, Email: gotok@sepia.ocn.ne.jp;

    Graduate Schools of Medicine and Frontier Biosciences, Osaka University, Toyonaka City, Osaka 560-0043, Japan, Email: ohira@space.hss.osaka-u.ac.jp;

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