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首页> 外文会议>Progress in functional amino acids and carbohydrates for animal production >Effects of dietary protein levels and genetic background on growth and carcass performance, serum parameter, enzyme and transcription factor mRNA expression in subcutaneous adipose tissues in swine
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Effects of dietary protein levels and genetic background on growth and carcass performance, serum parameter, enzyme and transcription factor mRNA expression in subcutaneous adipose tissues in swine

机译：日粮蛋白质水平和遗传背景对猪皮下脂肪组织生长和car体性能，血清参数，酶和转录因子mRNA表达的影响

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摘要

This study was mainly to investigate the effects of dietary crude protein (CP) levels on growth and carcass performance, serum parameter and the mRNA expression of enzymes and transcription factors in subcutaneous adipose tissues in obese and lean swine. Twenty seven obese-type Ningxiang breed fattening pigs and thirty six lean-type hybrid (Duroc × Landrace × Yorkshire) fattening pigs were randomly allocated to three by two groups, fed low, middle and high CP diets respectively for a 46-day period. The CP levels in the diets were 10.41% (low), 12.91% (middle) and 15.43% (high) for Ningxiang; 12.91% (low), 15.43% (middle) and 19.66% (high) for the hybrid according to their own standard maintenance requirement, respectively. All diets were identical in calories. On day 46, blood samples were collected for analysis of serum lipoprotein, total cholesterol, triglycerides and leptin levels. Following the blood collection, 4 pigs from each group were killed and subcutaneous adipose tissues were collected for the analysis of mRNA expression of lipogenic and lipolytic enzymes and transcription factors. Results indicated that dietary CP levels had no significant effect on growth and carcass performance, serum parameter and mRNA expression of lipogenic and lipolytic key enzymes and transcription factors except leptin and sterol regulatory element-binding protein-1c (SREBP-1c). Increases in dietary CP levels resulted in the increase of leptin and decrease of SREBP-1c mRNA expression in subcutaneous adipose tissues. However, there were marked differences in growth and carcass performance, serum parameter and mRNA expression of lipogenic and lipolytic key enzymes and transcription factors between the two breed. The percentage of fat and feed gain ratio, serum triglycerides, total cholesterol, low density lipoprotein and leptin content and the levels of hormone-sensitive lipase (HSL), lipoprotein lipase (LPL) and peroxisome proliferator-activated receptor-γ (PPARγ) mRNA in subcutaneous adipose tissues were significantly higher in Ningxiang pigs. And the average fat thickness was positively correlated with the abundance of HSL, LPL, leptin, PPARγ and liver X receptor-α (LXRα) mRNA in subcutaneous adipose tissues. Furthermore, leptin was positively correlated with LPL and LXRα, and was negatively correlated with SREBP-1c of mRNA expresstion in subcutaneous adipose tissues. These findings suggest that obese and lean genotype fattening pigs have distinct manners in lipid metabolism, the Ningxiang deposited higher energy than hybrid. And some lipid key enzymes (HSL, LPL) and transcription factors (PPARγ, SREBP-1c, LXRα) may participate in the regulation of lipid metabolism via leptin signaling. Dietary protein may indirectly act on these enzymes and transcription factors to influence the subcutaneous lipid metabolism.
机译：这项研究的主要目的是研究日粮粗蛋白（CP）水平对肥胖和瘦肉猪皮下脂肪组织的生长和car体性能，血清参数以及酶和转录因子的mRNA表达的影响。将27只肥胖型宁乡品种育肥猪和36只瘦型杂种（Duroc×Landrace×Yorkshire）育肥猪随机分为三组，每组分别饲喂低CP，中CP和高CP日粮，持续46天。宁乡县的日粮中CP水平分别为10.41％（低），12.91％（中）和15.43％（高）。根据自己的标准维护要求，混合动力车分别为12.91％（低），15.43％（中）和19.66％（高）。所有饮食的卡路里均相同。在第46天，收集血液样品以分析血清脂蛋白，总胆固醇，甘油三酸酯和瘦素水平。采血后，每组4只猪被处死，皮下脂肪组织被收集，用于分析脂肪和脂肪分解酶及转录因子的mRNA表达。结果表明，除了瘦素和固醇调节元件结合蛋白1c（SREBP-1c），日粮CP水平对生长期，car体性能，血清参数和脂生性，脂解性关键酶和转录因子的mRNA表达无明显影响。饮食中CP水平的增加导致皮下脂肪组织中瘦素的增加和SREBP-1c mRNA表达的减少。然而，两个品种之间的生长和car体性能，血清参数和生脂，脂解关键酶和转录因子的mRNA表达存在明显差异。脂肪和饲料增重比，血清甘油三酸酯，总胆固醇，低密度脂蛋白和瘦素含量的百分比以及激素敏感性脂酶（HSL），脂蛋白脂酶（LPL）和过氧化物酶体增殖物激活受体-γ（PPARγ）mRNA的水平宁乡猪皮下脂肪组织中的脂肪含量显着升高。并且平均脂肪厚度与皮下脂肪组织中HSL，LPL，瘦素，PPARγ和肝X受体α（LXRα）mRNA的含量呈正相关。此外，瘦素与LPL和LXRα呈正相关，与皮下脂肪组织中mRNA表达的SREBP-1c呈负相关。这些发现提示，肥胖和瘦肉型育肥猪的脂质代谢方式不同，宁乡猪比杂种猪的能量更高。一些脂质关键酶（HSL，LPL）和转录因子（PPARγ，SREBP-1c，LXRα）可能通过瘦素信号传导参与脂质代谢的调控。膳食蛋白可能间接作用于这些酶和转录因子，从而影响皮下脂质代谢。

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