• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Progress In Electromagnetic Research Symposium >Impact of electromagnetic field generated by mobile phone on prooxidant-antioxidant balance in testes of rats
【24h】

Impact of electromagnetic field generated by mobile phone on prooxidant-antioxidant balance in testes of rats

机译：手机产生的电磁场对大鼠睾丸中抗氧化剂和抗氧化剂平衡的影响

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

At the present stage of development of civilization human population is continuously exposed to different forms of electromagnetic field, especially that of radio frequency, generated by mobile phones. The long-term exposure to electromagnetic fields, can cause disturbances of the prooxidant/antioxidant balance in living organism and induce oxidative stress. In this study the impact of whole-body exposure to electromagnetic field with a frequency of 900MHz generated by mobile phone on prooxidant/antioxidant balance in the testes of male rats was estimated, by means of analysis of the contents of markers of membrane lipid peroxidation and oxidative stress: malone dialdehyde (MDA) and total oxidant capacity (TOC), respectively, the activity of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST), as well as the contents of marker of the activity of non-enzymatic antioxidant system: total antioxidant capacity (TAC), in homogenates of the testes. The experiment was performed on 20 male Wistar rats, in mean age of 10 weeks, divided into 2 equal groups (consisting of 10 animals), subjected to long-term exposure to electromagnetic field or to sham-exposure, respectively. Rats from examined group were exposed for 28 succeeding days to electromagnetic field with frequency of 900MHz generated by mobile phone Nokia 5110, that was turned on every 1/2 hour by 8 hours daily and emitted signal for 15 s. The mean value of power density of the electromagnetic field registered during initializing of connection was 85.3 μW/m2, and during lasting connection was 17.0 μW/m2. Rats from control group were exposed for 28 succeeding days to sham-exposure, during which they stayed in identical as exposed animals environmental conditions, excluding the influence of electromagnetic field. During the exposure the mobile phone was placed under a cage with animals. After the end of a cycle of 28 daily exposures to electromagnetic field or sham-exposures (control rats), animals were starved by 24 hours, then anaesthetized and next both testes were taken. In the homogenates prepared from the obtained testes the contents of MDA, TOC and TAC, as well as the activity of SOD, CAT, GPx, GR and GST were measured. The biochemical analyses were performed by means of routine spectrophotometric and kinetic methods. As a result of a cycle of repeated exposures, in electromagnetic field-exposed group of rats a significant decrease in the contents of MDA, a significant increase in the contents of TOC, as well as a significant increase in the activity of SOD, GR and GST in testicular tissue homogenates were observed, as compared to control sham-exposed rats. No significant differences in the activity of CAT and GPx, as well as in the contents of TAC in testicular tissue homogenates were noticed between both groups of rats. On the basis of the obtained results it was found, that 4-week lasting exposure of rats to electromagnetic field with physical parameters generated by mobile phone working in a frequency range of 900 MHz, causes in testes a significant increase of oxidative stress without significant changes of lipid peroxidation process intensity, as well as a stimulation of the activity of some antioxidant enzymes without changes in the activity of non-enzymatic antioxidant system.
机译：在文明发展的现阶段，人们不断暴露于手机产生的不同形式的电磁场，尤其是射频电磁场。长期暴露于电磁场中可能会破坏活生物体中的抗氧化剂/抗氧化剂平衡，并引起氧化应激。在这项研究中，通过分析膜脂质过氧化和氧化膜标记物的含量，估算了手机在900MHz频率下全身暴露于电磁场中对雄性大鼠睾丸中抗氧化剂/抗氧化剂平衡的影响。氧化应激：马龙二醛（MDA）和总氧化剂容量（TOC），抗氧化酶的活性：超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx），谷胱甘肽还原酶（GR）和谷胱甘肽S-转移酶（GST）以及非酶抗氧化系统活性标记的内容：睾丸匀浆中的总抗氧化能力（TAC）。实验是对平均年龄为10周的20只雄性Wistar大鼠进行的，将其分为两组，分别由10只动物组成，分别长期暴露于电磁场或假暴露。受测组的大鼠连续28天暴露于手机诺基亚5110产生的900MHz频率的电磁场中，该电磁场每1/2小时打开一次，每天8小时，并发射信号15 s。连接初始化期间记录的电磁场的功率密度平均值为85.3μW/ m2，持久连接期间为17.0μW/ m2。对照组的大鼠连续连续28天进行假暴露，其间与暴露的动物环境条件保持相同，但不包括电磁场的影响。在曝光期间，将手机放置在有动物的笼子下。在每天28次暴露于电磁场或假接触的周期（对照大鼠）结束后，将动物饿死24小时，然后麻醉，然后取下两个睾丸。在由获得的睾丸制备的匀浆中，测量了MDA，TOC和TAC的含量以及SOD，CAT，GPx，GR和GST的活性。生化分析通过常规的分光光度法和动力学方法进行。由于重复暴露的循环，在暴露于电磁场的大鼠中，MDA含量显着下降，TOC含量显着增加，SOD，GR和SOD的活性也显着增加。与对照组假暴露大鼠相比，观察到睾丸组织匀浆中的GST。两组大鼠之间的CAT和GPx活性以及睾丸组织匀浆中TAC含量均无显着差异。根据获得的结果，发现大鼠持续暴露于电磁场中持续4周，其电磁参数是由工作在900 MHz频率范围内的手机产生的，其睾丸中的氧化应激显着增加，而无明显变化脂质过氧化过程的强度，以及刺激某些抗氧化酶的活性，而不会改变非酶抗氧化系统的活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号