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Chemotaxis and migration of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy

机译：定量相数字全息显微镜监测无标记的三维环境中癌细胞的趋化性和迁移

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We investigated the capabilities of digital holographic microscopy (DHM) for label-free quantification of the response of living single cells to chemical stimuli in 3D assays. Fibro sarcoma cells were observed in a collagen matrix inside 3D chemotaxis chambers with a Mach-Zehnder interferometer-based DHM setup. From the obtained series of quantitative phase images, the migration trajectories of single cells were retrieved by automated cell tracking and subsequently analyzed for maximum migration distance and motility. Our results demonstrate DHM as a highly reliable and efficient tool for label-free quantification of chemotaxis in 2D and 3D environments.

机译：我们研究了数字全息显微镜（DHM）在3D分析中对活体单细胞对化学刺激的反应进行无标签定量的功能。使用基于Mach-Zehnder干涉仪的DHM装置，在3D趋化性室内的胶原蛋白基质中观察到纤维肉瘤细胞。从获得的一系列定量相位图像中，通过自动细胞跟踪检索单个细胞的迁移轨迹，然后分析最大迁移距离和运动性。我们的结果表明，DHM是一种高度可靠且高效的工具，可用于在2D和3D环境中对趋化性进行无标签定量。

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《Quantitative phase imaging III》|2017年|1007412.1-1007412.6|共6页
会议地点 San Francisco(US)
作者
Bjoern Kemper; Juergen Schnekenburger; Steffi Ketelhut;
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作者单位

Biomedical Technology Center of the Medical Faculty, University of Muenster, Mendelstr. 17, D-48149 Muenster, Germany;

Biomedical Technology Center of the Medical Faculty, University of Muenster, Mendelstr. 17, D-48149 Muenster, Germany;

Biomedical Technology Center of the Medical Faculty, University of Muenster, Mendelstr. 17, D-48149 Muenster, Germany;

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正文语种 eng
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live cell imaging; living cell cultures; digital holographic microscopy; quantitative phase imaging; minimally-invasive; label-free; tumor cell biology; chemotaxis;

机译：活细胞成像；活细胞培养；数字全息显微镜；定量相成像微创无标签；肿瘤细胞生物学；趋化性;

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专利

1. Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy [J] . Bjorn Kemper, Andreas Bauwens, Angelika Vollmer, Journal of biomedical optics . 2010,第3期

机译：通过数字全息显微镜对内皮细胞进行无标记定量细胞分裂监测
2. Design and development of volume phase holographic grating based digital holographic interferometer for label-free quantitative cell imaging [J] . Applied optics . 2020,第12期

机译：基于体积相位全息光栅的无标记定量细胞成像的设计与开发
3. Label-free observation of three-dimensional morphology change of a single PC12 cell by digital holographic microscopy [J] . Mir T.A., Shinohara H. Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2012,第1期

机译：通过数字全息显微镜无标记观察单个PC12细胞的三维形态变化
4. Chemotaxis and migration of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy [C] . Bjoern Kemper, Juergen Schnekenburger, Steffi Ketelhut Conference on quantitative phase imaging III . 2017

机译：通过定量相位数字全息显微镜监测三维环境中癌细胞迁移和癌细胞的迁移
5. Quantitative Phase Retrieval with Digital In-line Holographic Microscopy [D] . ?D'Angelo, Joshua M. 2020

机译：数字在线全息显微镜定量相位检索
6. Three-dimensional quantitative phase imaging of blood coagulation structures by optical projection tomography in flow cytometry using digital holographic microscopy [O] . Hideki Funamizu, Yoshihisa Aizu 2019

机译：使用数字全息显微镜在流式细胞仪中通过光学投影层析成像技术对凝血结构进行三维定量相成像
7. Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration. [O] . Dubois, Frank, Yourassowsky, Catherine, Monnom, Olivier, 2006

机译：数字全息显微镜用于体外癌细胞迁移的三维动态分析。

Chemotaxis and migration of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy

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