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首页> 外文会议>Recent researches in modern medicine >Contributions to Investigation of Oral Biofilms Using Low-Vacuum Scanning Electron Microscopy
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Contributions to Investigation of Oral Biofilms Using Low-Vacuum Scanning Electron Microscopy

机译：低压扫描电子显微镜对口腔生物膜研究的贡献

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摘要

A number of 8 periodontally-involved extracted teeth and 3 titanium implants terminally affected by periimplantitis were evaluated of the morphology of the biofilm of the periodontal pockets with a modified protocol involving a simplified histologic sample preparation and a low-vacuum SEM examination method. The samples were carefully washed with saline solution, underwent fixation in modified Karnovsky solution (2.5% glutaraldehyde, 4% paraformaldehyde, 0.1M sodium cacodilate, pH7.2-7.4) and were dehydrated in alcohol series (70%, 95%, 100%). The samples were immediately examined under the low-vacuum SEM (INSPECT S, FEI, Hillsboro, OR, USA), at 80-250 Pa and 15kV. The low-vacuum inspection resulted in electrical charge-free images of the non-conductive or heavily-contaminated biofilm-covered surfaces. The low-vacuum SEM method avoids the sputtering of samples, the destruction of the biologic samples and the electrostatic charges. The low-vacuum SEM is a simple and cost-saving method, resulting in excellent quality biofilm images in contradistinction to conventional SEM that requires a complicate procedure, consisting in fixation of samples in glutaraldehyde and/or osmium tetroxide, followed by dehydration using either critical point drying with liquid CO2 or an alcohol series, and coating (sputtering) of biofilm with conductive metallic material (Au, Pa or Carbon). The microorganisms described by the SEM investigation were mostly coccoid forms, followed by rare rods and filaments. No spirilar shapes and spirochetes were observed during investigation.
机译：使用改良的方案，包括简化的组织学样品制备和低真空SEM检查方法，对8颗牙周受累的拔牙和3颗最终受到种植体周围炎终末影响的钛植入物进行了评估，评估了牙周袋生物膜的形态。样品用盐溶液仔细洗涤，在改良的Karnovsky溶液（2.5％戊二醛，4％多聚甲醛，0.1M椰油酸钠，pH7.2-7.4）中固定，并在乙醇系列中脱水（70％，95％，100％ ）。立即在80-250 Pa和15kV的低真空SEM（INSPECT S，FEI，Hillsboro，OR，美国）下检查样品。低真空检查会产生非导电或严重污染的生物膜覆盖表面的无电荷图像。低真空SEM方法避免了样品的溅射，生物样品的破坏和静电荷。低真空SEM是一种简单且节省成本的方法，与传统SEM相比，它需要高质量的生物膜图像，而传统SEM则需要复杂的过程，包括将样品固定在戊二醛和/或四氧化中，然后使用任意一种进行脱水用液态CO2或酒精系列进行点干燥，然后用导电金属材料（Au，Pa或Carbon）涂覆（溅射）生物膜。 SEM研究中描述的微生物主要为类球体形式，其次是稀有的杆和细丝。在研究过程中未观察到螺旋形和螺旋形。

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  • 来源
    《Recent researches in modern medicine》|2011年|p.379-384|共6页
  • 会议地点 Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB)
  • 作者

    STEFAN-IOAN STRATUL; DARIAN RUSU; ANDREEA DIDILESCU; LUMINITA NICA; AUREL RADUT/A; COSMIN LOCOVEI; MIRCEANICOARA;

  • 作者单位

    Department of Periodontolgy Victor Babes Medical University Timisoara, Romania;

    Department of Periodontolgy Victor Babes Medical University Timisoara, Romania;

    Department of Anatomy and Embryology Carol Davila University of Medicine Bucharest, Romania;

    Department of Endodontics Victor Babes Medical University Timisoara, Romania;

    Department of Materials and Manufacturing Engineering, POUTEHNICA University of Timisoara Bd. M. Viteazul 1, 300222 Timisoara, ROMANIA;

    Department of Materials and Manufacturing Engineering, POUTEHNICA University of Timisoara Bd. M. Viteazul 1, 300222 Timisoara, ROMANIA;

    Department of Materials and Manufacturing Engineering, POUTEHNICA University of Timisoara Bd. M. Viteazul 1, 300222 Timisoara, ROMANIA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    low vacuum SEM; periodontal pockets; periimplantitis; biofilm; glycocalyx; kamovsky solution.;

    机译：低真空SEM牙周袋种植体周围炎;生物膜糖萼kamovsky解决方案。;

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