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The observation research of the differences in cell death and reactive oxygen specises in the process of infecting Arabidopsis with avirulent strains

机译:无毒菌株感染拟南芥过程中细胞死亡和活性氧种类差异的观察研究

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Objective: To observe the differences of cell death and accumulation of reactive oxygen species (ROS) in the process of infecting Arabidopsis with avirulent Pseudomonas syringae pv. tomato DC3000 (avrB, avrRps4), it will be of great importance to research the role of plant disease resistance and defense response. Methods: Using WT, AtrbohD and AtrbohF mutant as experimental materials, we discuss the impact of cell death and ROS on the leaves of Arabidopsis infected with avirulent Pst DC3000 {avrB, avrRps4), observed by spectral analysis and visualized by DAB and trypan blue stain. Results: When infected with avirulent Pst DC3000, both WT and AtrbohF mutant line behaved resistance that exhibited burst of ROS and HR occur, limit senescence and pathogen induced chlorotic cell death. Paradoxically, AtrbohD mutant behaved susceptible characters that exhibited a small quantity of ROS accumulated and enhanced cell death. Conclusion: After infection of Arabidopsis with avirulent Pst DC3000, WT exhibited more ROS accumulation than AtrbohF, and AtrbohD eliminated the majority of total ROS production. Although both WT and AtrbohF mutant exhibited HR occur, enhanced cell death in AtrbohD mutant.
机译:目的:观察无毒丁香假单胞菌pv感染拟南芥过程中细胞死亡和活性氧(ROS)积累的差异。番茄DC3000(avrB,avrRps4),研究植物抗病性和防御反应的作用将具有重要意义。方法:以野生型,AtrbohD和AtrbohF突变体为实验材料,讨论细胞死亡和ROS对无毒Pst DC3000(avrB,avrRps4)感染的拟南芥叶片的影响,通过光谱分析观察并通过DAB和台盼蓝染色观察。结果:当感染无毒的Pst DC3000时,WT和AtrbohF突变株均表现出抗性,表现出ROS和HR爆发,限制了衰老和病原体诱导的绿藻细胞死亡。矛盾的是,AtrbohD突变体表现出易感特征,表现出少量的ROS积累并增加了细胞死亡。结论:用无毒的Pst DC3000感染拟南芥后,WT表现出比AtrbohF更多的ROS积累,而AtrbohD消除了大部分ROS产生。尽管WT和AtrbohF突变体均表现出HR,但在AtrbohD突变体中细胞死亡增加。

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