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Light stress effect and by nitrogen deficiency in plants of Petiveria alliacea measured with two chlorophyll fluorescence technique

机译:两种叶绿素荧光技术测定矮牵牛的光胁迫效应和氮不足

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The chlorophyll fluorescence was studied in Petiveria alliacea plants exposed to different nitrogen concentrations and light radiation, the response was measured by two different forms; a) measuring the photosynthetic efficiency with a pulse amplitude modulated fluorometro (PAM) emitted by a 650nm diode and b) measuring the fluorescence spectra caused by high power 452nm diode with a SD2000 spectrometer. It was find out that the photosynthetic efficiency decreased in the plants exposed to high radiance and low nitrogen. Two chlorophyll fluorescence peaks were observed on 684nm and 739nm, the intensities in this wavelengths are inversely related with the light radiance. The correlation between the FIR and photosynthetic efficiency was very strong (r~2= -0.809, p < < 0.01); this let us conclude that the fluorescence spectral analysis induced by the diode (excitation at 452nm) is an efficient technique to detect stress by high light intensity and nitrogen in P. Alliacea plants
机译:在暴露于不同氮浓度和光辐射下的矮牵牛中研究叶绿素荧光,通过两种不同形式测量其响应。 a)用650nm二极管发出的脉冲幅度调制的荧光光谱仪(PAM)测量光合作用效率,b)用SD2000光谱仪测量高功率452nm二极管引起的荧光光谱。发现在暴露于高辐射和低氮的植物中,光合作用效率降低。在684nm和739nm处观察到两个叶绿素荧光峰,该波长下的强度与光辐射成反比。 FIR与光合效率之间的相关性非常强(r〜2 = -0.809,p <<0.01);这使我们得出结论,由二极管诱导的荧光光谱分析(在452nm处激发)是一种有效的技术,能够通过高光强度和氮来检测假单胞菌植物中的胁迫

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