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首页> 外文会议>Second International Conference on Sustainable Agriculture for Food, Energy and Industry Vol.2 >Characterization of Backcross-Inbred Lines of Bread Wheat Using Protein and Isozyme Markers
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Characterization of Backcross-Inbred Lines of Bread Wheat Using Protein and Isozyme Markers

机译：利用蛋白质和同工酶标记表征面包小麦的回交自交系

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Traditional wheat varieties like C-306M10, KH-65, and C-591 have got genes for drought, salinity resistance and quality, respectively. However, they possess inherent shortcomings for being tall and cannot be accepted for commercial cultivation. The yield advantage associated with Norin-10 has been exploited particularly in Mexican wheat varieties and their derivatives emanating from crosses with indigenous type. However, inclusion of other dwarfing sources like S-948A1 (Rht_1& Rht_2) and Tordo (Rht_3) have not been exploited hitherto. A set of backcross inbred lines (BILs) has been developed and these exhibit significant differences in plant height and other traits. These lines were characterized using protein and isozyme markers. Electrophoretic protein patterns revealed considerable differences among parents and lines derived from them. Also there was considerable variation among lines. Of the three isozymes (SOD, POX and MDH) studied, Peroxidase isozyme revealed maximum polymorphism. These studies will be useful in registration of potential material for further use in wheat breeding.
机译：传统小麦品种（如C-306M10，KH-65和C-591）分别具有抗旱，耐盐碱和优质的基因。但是，它们具有很高的内在缺点，不能被商业化种植。与Norin-10相关的产量优势已被特别利用在墨西哥小麦品种及其衍生自本地品种杂交的衍生物中。但是，到目前为止，尚未利用其他矮人资源，例如S-948A1（Rht_1＆Rht_2）和Tordo（Rht_3）。已经开发出一套回交自交系（BIL），它们在植物高度和其他性状上表现出显着差异。这些品系使用蛋白质和同工酶标记进行表征。电泳蛋白质图谱显示亲本和衍生自它们的品系之间存在显着差异。各行之间也存在很大差异。在研究的三种同工酶（SOD，POX和MDH）中，过氧化物酶同工酶显示出最大的多态性。这些研究将有助于潜在材料的注册，以进一步用于小麦育种。

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