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首页> 外文会议>The Second Asian symposium on pharmaceutical sciences and technology >A pre-column derivatization-HPLC method for determination of oxalipIatin(L-OHP)content and entrapment efficiency in nanoparticles
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A pre-column derivatization-HPLC method for determination of oxalipIatin(L-OHP)content and entrapment efficiency in nanoparticles

机译：柱前衍生高效液相色谱法测定纳米颗粒中草酸化磷脂（L-OHP）的含量和包封率

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Purpose: To develop an improved -HPLC method for the determination of oxaliplatin(L-OHP) content and entrapment efficiency in nanoparticles. Methods: After derivatization with sodium diethyldithiocarbamate (DDTC), Pt(DDTC)2 and diazepam (the internal standard) was separated on C18 column (250 mm×4.6 mm, 5 urn) at 30°Cusing a mobile phase consisting of methol and water (75:25, v/v) at flow rate of 1 ml-min-1; The method was validated with respect to selectivity, linearity, precision and accuracy, limit of quantification and stability. The ultrafiltration was employed to separate the free L-OHP from nanoparticles to calculate the entrapment efficiency. Results: The calibration curve of L-OHP was linear in the concentration range of 5.26-31.53 ugml-1. (r= 0.9997) with the limit of quantification (LOQ) of 0.42 ug/mL. The variation coefficients were not more than 1.63%. The mean recoveries was from 98.29% to 100.45%. The content and the encapsulation efficiency of L-OHP nanoparticles were 1.69mg/ml and 63%, respectively. Conclusion:The present method can be used to determine L-OHP content in prepared nanoparticles.as well as entrapment efficiency.
机译：目的：建立改进的高效液相色谱法测定纳米颗粒中奥沙利铂（L-OHP）的含量和包封率。方法：用二乙基二硫代氨基甲酸钠（DDTC）衍生化后，在30°C的C18色谱柱上（250 mm×4.6 mm，5 n）分离Pt（DDTC）2和地西epa（内标），使用由甲醇和水组成的流动相（75:25，v / v），流速为1 ml-min-1；该方法在选择性，线性，精密度和准确性，定量限和稳定性方面得到了验证。使用超滤从纳米颗粒中分离出游离的L-OHP，以计算包封率。结果：L-OHP的校正曲线在5.26-31.53 ugml-1的浓度范围内是线性的。 （r = 0.9997），定量限（LOQ）为0.42 ug / mL。变异系数不超过1.63％。平均回收率从98.29％增至100.45％。 L-OHP纳米粒子的含量和包封效率分别为1.69mg / ml和63％。结论：本方法可用于测定制备的纳米颗粒中L-OHP的含量以及包封率。

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