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首页> 外文会议>Second European Workshop on Exo-Astrobiology Sep 16-19, 2002 Graz, Austria >THE FIRST CRYSTALLIZATION OF THE OUTER SURFACE (S-LAYER) GLYCOPROTEIN OF THE MESOPHILIC BACTERIUM BACILLUS SPHAERICUS AND THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS
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THE FIRST CRYSTALLIZATION OF THE OUTER SURFACE (S-LAYER) GLYCOPROTEIN OF THE MESOPHILIC BACTERIUM BACILLUS SPHAERICUS AND THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS

机译：嗜热芽孢杆菌和超嗜热古细菌METHANOTHERMUS FERVIDUS的外表面（S-层）糖蛋白的首次结晶

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摘要

Since a few years our interest is focused on the determination of the 3D crystal structure of S-layer (glyco-) proteins from methanogenic Archaea of the genus Methanothermus and mesophilic, thermophilic and extremely thermophilic species of the genus Methano-coccus in order to obtain a better understanding of the principles of molecular stabilization of extremely thermophilic cell surface proteins. Crystals from the S-layer glycoprotein of Methanothermus fervidus were grown under microgravity conditions during the flight STS-95 of the space shuttle Discovery. We extended our research also to the domain Bacteria in order to find out whether the stabilization of these thermophilic S-layer proteins is based on the same principles as in the domain Archaea. In this case members of the genus Bacillus were chosen because the species of this genus are closely related to each other as it is the case with the Methanococci and mesophilic as well as thermophilic species exist. In the meantime, we were able to grow also crystals of the S-layer glycoprotein of Bacillus sphaericus.
机译：几年来，我们的兴趣集中在确定甲烷甲烷球菌属的产甲烷古生菌和甲烷球菌属的嗜温，嗜热和极嗜热菌种的S层（糖基）蛋白的3D晶体结构上，从而获得对极端嗜热细胞表面蛋白分子稳定原理的更好理解。在发现号航天飞机STS-95的飞行过程中，在极度重力作用下，生长了费氏甲烷甲烷嗜热菌S层糖蛋白的晶体。我们也将研究扩展到细菌域，以发现这些嗜热S层蛋白的稳定化是否基于与古细菌域相同的原理。在这种情况下，选择了芽孢杆菌属的成员，因为该属的物种彼此紧密相关，就像甲烷球菌一样，并且存在嗜温和嗜热物种。同时，我们还能够生长球形芽孢杆菌S层糖蛋白的晶体。

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