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THz spectroscopy based high sensitivity measurement of protein using a metal mesh device

机译:使用金属筛网设备基于THz光谱的蛋白质高灵敏度测量

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A label-free bioaffinity sensor working in terahertz (THz) region with a nitrocellulose membrane filter was demonstrated, which is based on the resonant transmission phenomenon and the dip in the spectra of the metal mesh device. By using this sensor, we succeeded in the highly sensitive detection of small amounts of protein avidin-biotin complex. A distinct change of transmittance caused by shift of the transmission dip was observed for 8 ng/mm2 (74 fmol) of horseradish peroxidase (HRP) labeled avidin. The sensing method has broad utility for many reactions on the membrane filter as a simple and rapid sensor.
机译:展示了一种基于硝酸纤维素膜滤光片的,在太赫兹(THz)区域工作的无标记生物亲和力传感器,该传感器基于共振透射现象和金属网状器件光谱的下降。通过使用该传感器,我们成功地高灵敏度检测了少量的蛋白质亲和素-生物素复合物。对于8 ng / mm2(74 fmol)的辣根过氧化物酶(HRP)标记的亲和素,观察到由透射率下降引起的透射率明显变化。该感测方法作为简单而快速的传感器,对于膜滤器上的许多反应具有广泛的用途。

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