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Phage-Based Biomolecular Filter for the Capture of Bacterial Pathogens in Liquid Streams

机译:基于噬菌体的生物分子过滤器,用于捕获液体流中的细菌病原体

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This paper investigates a phage-based biomolecular filter that enables the evaluation of large volumes of liquids for the presence of small quantities of bacterial pathogens. The filter is a planar arrangement of phage-coated, strip-shaped magnetoelastic (ME) biosensors (4 mm × 0.8 mm × 0.03 mm), magnetically coupled to a filter frame structure, through which a liquid of interest flows. This "phage filter" is designed to capture specific bacterial pathogens and allow non-specific debris to pass, eliminating the common clogging issue in conventional bead filters. ANSYS Maxwell was used to simulate the magnetic field pattern required to hold ME biosensors densely and to optimize the frame design. Based on the simulation results, a phage filter structure was constructed, and a proof-in-concept experiment was conducted where a Salmonella solution of known concentration were passed through the filter, and the number of captured Salmonella was quantified by plate counting.
机译:本文研究了一种基于噬菌体的生物分子过滤器,该过滤器能够评估大量液体中是否存在少量细菌病原体。过滤器是噬菌体涂层的条形磁弹性(ME)生物传感器(4毫米×0.8毫米×0.03毫米)的平面布置,磁耦合至过滤器框架结构,感兴趣的液体流过该框架。这种“噬菌体过滤器”旨在捕获特定的细菌病原体,并允许非特定的碎片通过,从而消除了常规珠粒过滤器中常见的堵塞问题。 ANSYS Maxwell用于模拟密集放置ME生物传感器所需的磁场模式并优化框架设计。基于模拟结果,构建了噬菌体过滤器结构,并进行了概念验证实验,其中已知浓度的沙门氏菌溶液通过过滤器,并通过平板计数对捕获的沙门氏菌数进行定量。

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