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Effects of Food Surface Topography on Phage-Based Magnetoelastic Biosensor Detection

机译：食物表面形貌对基于噬菌体的磁弹性生物传感器检测的影响

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Phage-based magnetoelastic (ME) biosensors have proven useful in rapidly and inexpensively detecting food surface contamination. These biosensors are wireless, mass-sensitive biosensors and can be placed directly on food surfaces to detect the presence of target pathogens. Previously, millimeter-scale strip-shaped ME biosensors have been used to demonstrate direct detection of Salmonella Typhimurium on various fresh produce surfaces, including tomatoes, shell eggs, watermelons, and spinach leaves. Since the topography of these produce surfaces are different, and the biosensor must come into direct contact with Salmonella bacteria, food surfaces with large roughness and curvatures (e.g., spinach leaf surfaces) may allow the bacteria to avoid direct contact, thereby avoiding detection. The primary objective of this paper is, hence, to investigate the effects of food surface topography on the detection capabilities of the biosensors. Spinach leaf surfaces were selected as model surfaces, and detection experiments were conducted with differently sized biosensors (2 mm, 0.5 mm, and 150 μm in length). Spinach leaf roughness and curvatures of both adaxial (top) and abaxial (underside) surfaces were measured using a confocal laser scanning microscope. The experimental results showed that in spinach as the sensor was made smaller, the physical contact between the biosensors and bacteria were improved. Smaller sensors thereby enhance detection capabilities. When proper numbers of biosensors are used, micron-scale biosensors are anticipated to yield improved limits of detection over previously investigated millimeter-scale biosensors.
机译：基于噬菌体的磁弹性（ME）生物传感器已被证明可用于快速，廉价地检测食品表面污染。这些生物传感器是无线的，对质量敏感的生物传感器，可以直接放置在食品表面上以检测目标病原体的存在。以前，毫米级的条形ME生物传感器已被用于证明在各种新鲜农产品表面上直接检测鼠伤寒沙门氏菌，包括西红柿，带壳蛋，西瓜和菠菜叶。由于这些产品表面的形貌不同，并且生物传感器必须与沙门氏菌直接接触，因此具有较大粗糙度和曲率的食物表面（例如菠菜叶表面）可能会使细菌避免直接接触，从而避免检测。因此，本文的主要目的是研究食物表面形貌对生物传感器检测能力的影响。选择菠菜叶表面作为模型表面，并使用不同尺寸的生物传感器（长度分别为2 mm，0.5 mm和150μm）进行检测实验。使用共聚焦激光扫描显微镜测量菠菜叶片的粗糙度（正面（顶部）和背面（背面））和曲率。实验结果表明，在菠菜中，随着传感器的变小，生物传感器与细菌之间的物理接触得以改善。较小的传感器从而增强了检测能力。当使用适当数量的生物传感器时，与先前研究的毫米级生物传感器相比，微米级生物传感器有望产生更高的检测限。

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